Comparison of somatic cell count in raw milk by four staining methods

Authors

  • Kelvinson Fernandes Viana
  • Briza Ferreira Setubal
  • Vivian Araújo Mendes
  • Pricila Aparecida Grasse Pietralonga
  • Marcos Santos Zanini

DOI:

https://doi.org/10.21708/avb.2010.4.1.1586

Abstract

The somatic cell count (SCC) determines the amount of leukocytes and epithelial cells present in milk. In case of inflammation (subclinical or clinical), there is a considerable increase in SCC, especially neutrophils. It is important to quantify and qualify the cell types present to know the degree of inflammation and characterized as acute or chronic. The objective was to test four staining methods of somatic cells; Broadhurst-Paley, hematoxylin-eosin stain Panoptic ® and May Grunwald-Giemsa by light microscopy and determine the most suitable for our laboratory conditions. Considering the available data, was elected the association of eosin staining and methylene blue (May-Grunwald Giemsa) with which they obtained results ranging from 60,000 to 58,690,000 cells/ml in different samples of bovine milk. It was not possible to quantify the cells by the other three staining methods due to morphological changes exacerbated them. Key words: somatic cell, color, milk

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Published

2010-04-23

Issue

Section

Original Articles / Artigos de Pesquisa

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