Influence of remating on sterile insect technique in Ceratitis capitata (Diptera: Tephritidae): a molecular approach

Vilmara Pereira Barboza; Beatriz Aguiar Jordão Paranhos; Juvenal Cordeiro Silva junior

doi:10.1590/1983-21252023v36n308rc

Autores

Vilmara Pereira Barboza Department of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Jequié, BA https://orcid.org/0000-0001-9133-162X
Beatriz Aguiar Jordão Paranhos Laboratory of Entomology, Embrapa Semiárido, Petrolina, PE https://orcid.org/0000-0001-6814-3831
Juvenal Cordeiro Silva junior Department of Biological Sciences, Universidade Estadual do Sudoeste da Bahia, Jequié, BA https://orcid.org/0000-0003-1730-0926

DOI:

https://doi.org/10.1590/1983-21252023v36n308rc

Palavras-chave:

Criação massal. Espermateca. Fruticultura. Marcadores genéticos. Moscamed.

Resumo

A mosca-das-frutas do Mediterrâneo, Ceratitis capitata (Diptera: Tephritidae), é uma das espécies mais prejudiciais à horticultura mundial, portanto requer programas de supressão ou erradicação. A técnica do inseto estéril é amplamente utilizada para o controle autocida de insetos nocivos, pois não libera agentes exóticos no meio ambiente nem introduz novos materiais genéticos nas populações existentes. Em 2005, a biofábrica Moscamed Brasil importou a cepa Vienna 8 C. capitata da Agência Internacional de Energia Atômica (IAEA); essa ação exigiu testes genéticos das características biológicas da linhagem. O objetivo deste estudo foi discriminar machos de populações silvestres de tsl Viena 8 usando marcadores moleculares, e inferir sobre qual linhagem masculina era dominante em condição de recópula. Quatro cruzamentos, cada um com 100 machos e 100 fêmeas, foram realizados: fêmea selvagem × macho selvagem; fêmea selvagem × macho tsl Viena 8; fêmea selvagem × macho selvagem, seguido por recópula com machos tsl Vienna 8 após 48 h, e fêmea selvagem × machos tsl Vienna 8, seguido por recópula com machos selvagens após 48 h. Os resultados mostraram que a cepa tsl Vienna 8 é compatível com fêmeas selvagens da região do Vale do São Francisco e que esses machos podem transferir com sucesso seus espermatozóides para a espermateca das fêmeas. Quanto à recópula, o esperma do segundo macho precedeu o primeiro. Com base nesses achados, o sucesso da técnica de insetos estéril pode ser reduzido, já que a progênie de uma fêmea que recopulou com um macho selvagem pode ser viável.

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