Estudo de toxicidade aguda de galactomannas bioativas de sementes de duas leguminosas não tradicionais

Autores

  • Fabrícia da Cunha Jácome Marques Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-1560-0741
  • Francisco Glerison da Silva Nascimento Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-2481-5733
  • Dayanne Terra Tenório Nonato Universidade Federal do Rio Grande do Norte, Natal, RN, Brazil https://orcid.org/0000-0001-9477-1866
  • Ana Maria Sampaio Assreuy Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-2323-5385
  • Edna Maria Camelo Chaves Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0001-9658-0377
  • Gislei Frota Aragão Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-0689-8371
  • Pedro Marcos Gomes Soares Department of Morphology, Universidade Federal do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0003-0606-2539
  • Rondinelle Ribeiro Castro Superior Institute of Biomedical Sciences, Universidade Estadual do Ceará, Fortaleza, CE, Brazil https://orcid.org/0000-0002-9399-4109

DOI:

https://doi.org/10.1590/1983-21252025v3812704rc

Palavras-chave:

Polissacarídeo. Galactomanana. Segurança. Flor-de-pavão. Flamboiã.

Resumo

Fontes não tradicionais de galactomananas são amplamente cultivadas na Região Nordeste do Brasil, como as sementes das espécies ornamentais Caesalpinia pulcherrima (flor-de-pavão) e Delonix regia (flamboiã). As galactomananas GM-CP e GM-DR, extraídas respectivamente dessas sementes, estão sendo estudadas como potenciais agentes terapêuticos, mas avaliações sistemáticas sobre a toxicidade aguda das mesmas ainda não foram relatadas. Grupos de três ratas receberam GM-CP ou GM-DR oral (300 mg kg-1), enquanto um grupo controle recebeu veículo (solução salina). Como não houve letalidade, outros grupos receberam doses de 2000 mg kg-1, o que também não causou letalidade. A coleta de órgãos e amostras de sangue foi feita no dia 14. A hipernocicepção mecânica e o influxo de células inflamatórias foram medidos em outros grupos que receberam doses intra-articulares de GM-CP ou GM-DR (200 µg, n = 5 por grupo). Ambas as galactomananas não evocaram alterações fisiológicas/comportamentais ou inflamação articular. Como a DL50 não foi inferior a 2000 mg kg-1, tais polissacarídeos podem ser alocados na classe 5 do Sistema Globalmente Harmonizado para Classificação e Rotulagem de Produtos Químicos.

Downloads

Referências

ALSULIAM, S. M. et al. Fenugreek seed galactomannan aqueous and extract protects against diabetic noephopathy and liver damage by targeting NF-kB and Keap1/Nrf2 axis. Toxics, 10: 362, 2022.

BRINGEL, P. H. S. F. et al. The lectin isolated from the alga Hypnea cervicornis promotes antinociception in rats subjected to zymosan-induced arthritis: involvement of cGMP signalization and cytokine expression. Inflammation, 43: 1446-1454, 2020.

BURITI, F. C. A. et al. Characterization of partially hydrolysed galactomannan from Caesalpinia pulcherrima seeds as a potential dietary fibre. Food Hydrocolloids, 35: 512-521, 2014.

CHEL-GUERRERO, L. et al. Films properties of QPM corn starch with Delonix regia seed galactomannan as an edible coating material. International Journal of Biological Macromolecules, 255: 128408, 2024.

CUNHA, A. P. et al. Polysaccharides from Caesalpinia ferrea seeds - Chemical characterization and anti-diabetic effects in Wistar rats. Food Hydrocolloids, 65: 68-76, 2017.

CERQUEIRA, M. A. et al. Extraction, purification and characterization of galactomannans from non-traditional sources. Carbohydrate Polymers, 75: 418-414, 2009.

DESHPANDE, P., MOHAN, V., THAKURDESAI, P. Preclinical safety evaluation of low molecular weight galactomannans based standardized fenugreek seeds extract. EXCLI Journal, 15: 446-459, 2016.

JAIN, N. et al. Haemato-biochemical profile following sub acute toxicity of malathion in male albino rats. Pharmacology Online, 2: 500-506, 2009.

JOHNSON, W. et al. Safety Assessment of Galactomannans as Used in Cosmetics. International Journal of Toxicology, 34: 35-65, 2015.

JOSEPH, A. et al. Safety assessment of a fenugreek dietary fiber-based formulation of capsaicinoids-rich red chili (Capsicum annum) extract (Capsifen®): Acute and sub-chronic studies. Toxicology Reports, 7: 602-609, 2020.

LIJU, V. B. et al. Enhanced bioavailability and safety of curcumagalactomannosides as a dietary ingredient. Food and Function, 6: 276-286, 2015.

LIMA, E. L. et al. Injectable hydrogel based on dialdehyde galactomannan and N-succinyl chitosan: a suitable platform for cell culture. Journal of Materials Science: Materials in Medicine, 31: 5-13, 2019.

LIMA, I. C. et al. Galactomannan of Delonix regia seeds modulates cytokine expression and oxidative stress eliciting anti-inflammatory and healing effects in mice cutaneous wound. International Journal of Biological Macromolecules, 203: 342-349, 2022.

MALONE, M. H. Pharmacological approaches to natural product screening and evaluation. In: WAGNER, H.; WOLFF, P. M. (Eds.). New Natural Products and Plant Drugs with Pharmacological, Biological or Therapeutical Activity. Berlin Heldelberg New York: Springer-Verlag, 1977. v. 1, cap. 2, p. 23-53.

MARQUES, F. C. J. et al. Galactomannan from the seeds of Caesalpinia pulcherrima prevents indomethacin-induced gastrointestinal damage via neutrophil migration. International Journal of Biological Macromolecules, 141: 68-75, 2019.

MODI, A. et al. Delonix regia: historic perspectives and modern phytochemical and pharmacological researches. Chinese Journal of Natural Medicines, 14: 31-39, 2016.

NASCIMENTO, F. G. S. et al. Galactomannan of Delonix regia seeds reduces nociception and morphological damage in the rat model of osteoarthritis induced by sodium monoiodoacetate. Naunyn-Schmiedeberg’s Archives of Pharmacology, 394: 491-501, 2021.

OECD.Test No. 423: Acute Oral toxicity - Acute Toxic Class Method, OECD Guidelines for the Testing of Chemicals, Paris: OECD Publishing, 2002, Section 4. Paris.

OGUNJIMI, A. T. et al. Hydrophilic polymeric nanoparticles prepared from Delonix galactomannan with low cytotoxicity for ocular drug delivery. Carbohydrate Polymers, 157: 1065-1075, 2017.

PAWAR, A. H.; LALITHA, K. G. Toxicological Evaluation of Gum (Galactomannans) Isolated from Senna tora Seeds. Journal of Drug Metabolism and Toxicology, 6: 1-5, 2015.

SHARMA, P. et al. A comprehensive review on leguminous galactomannans: structural analysis, functional properties, biosynthesis process and industrial applications

Critical Reviews in Food Science and Nutrition, 62 : 443-465, 2022.

SURYAWANSHI, S. R. et al. Bioavailability enhancement of ondansetron after nasal administration of Caesalpinia pulcherrima-based microspheres. Drug Delivery, 22: 894-902, 2015.

UN - United Nations Globally Harmonized System of Classification and Labelling of Chemicals (GHS). 8 ed. New York and Geneva: UN, 2019.

WANG, R. et al. A contribution to improve barrier properties and reduce swelling ratio of κ-carrageenan film from the incorporation of guar gum or locust bean gum. Polymers, 15: 1751, 2023.

WANG, Z. et al. Estimation of the normal range of blood glucose in rats. Wei Sheng Yan Jiu, 2: 133-140, 2010.

Downloads

Publicado

24-09-2024

Edição

Seção

Artigo Científico