MEDICINAL PLANTS FROM BRAZILIAN CAATINGA: ANTIBIOFILM AND ANTIBACTERIAL ACTIVITIES AGAINST Pseudomonas aeruginosa
Palavras-chave:
Biofilm. Anadenanthera colubrina. Commiphora leptophloeos. Myracrodruoun urundeuva. Pityrocarpa moniliformis. Traditional medicine.Resumo
The Caatinga biome covers a vast area in northeastern Brazil and presents a high level of biodiversity. It is known that about 400 plant species are used by semi-arid local communities for medical purposes. Based on ethnopharmacological reports, this study aims to screen 24 species from Caatinga regarding the ability to prevent biofilm formation and to inhibit the growth of Pseudomonas aeruginosa - a major opportunistic human pathogen and an important causative agent of morbidity and mortality. The effects of aqueous extracts, at 0.4 and 4.0 mg mL-1, on biofilm formation and on growth of P. aeruginosa ATCC 27853 were studied using the crystal violet assay and the OD600 absorbance, respectively. The most active extracts were analyzed by thin-layer chromatography and high performance liquid chromatography. Our investigation pointed extracts of four species with potential application for the control of P. aeruginosa: Anadenanthera colubrina (Vell.) Brenan, Commiphora leptophloeos (Mart.) J.B. Gillett, Myracrodruoun urundeuva Allemão, whose antibiofilm effects (89%, 56% and 79% inhibition of biofilm, respectively) were associated with complete inhibition of bacterial growth, and Pityrocarpa moniliformis (Benth.) Luckow & R.W. Jobson, which were able avoid 68% of biofilm formation and inhibited 30% bacterial growth. The qualitative phytochemical analyses reveal the complexity of the samples as well as the presence of compounds with high molecular weight.Downloads
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Referências
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ARAÚJO, T. A. S. et al. A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology, v. 120, n. 1, p. 72-80, 2012.
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CEGELSKI, L. et al. The biology and future prospects of antivirulence therapies. Nature Reviews Microbiology, v. 6, n. 11, p. 17-27, 2008.
DING, X. et al. Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm. Journal of Medical Microbiology, v. 60, n. 12, p. 1827-1834, 2011.
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HERRERA, R. M. et al. Antimicrobial activity of extracts from plants endemic to the Canary Islands. Phytotherapy Research, v. 10, n. 4, p. 364-66, 1996.
KOKOSKA, L. et al. Screening of some Siberian medicinal plants for antimicrobial activity. Journal of Ethnopharmacology, v. 82, n. 1, p. 51-53, 2002.
KONÉ, W. M. et al. Traditional medicine in north Côte-d'Ivoire: screening of 50 medicinal plants for antibacterial activity. Journal of Ethnopharmacology, v. 93, n. 1, p. 43-49, 2004.
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RYBTKE, M. T. et al. The implication of Pseudomonas aeruginosa biofilms in infections. Inflammation & Allergy-Drug Targets, v. 10, n. 2, p. 141-157, 2011.
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SIQUEIRA, C. F. et al. Levels of tannins and flavonoids in medicinal plants: evaluating bioprospecting strategies. Evidence-Based Complementary and Alternative Medicine, Article ID 434782, 2012.
SOULI, M.; GALANI, I.; GIAMARELLOU, H. Emergence of extensively drug-resistant and pandrug-resistant Gram-negative bacilli in Europe. Euro Surveillance, v. 13, n. 47, p. 1-11, 2008.
STRATEVA, T.; YORDANOV, D. Pseudomonas aeruginosa - a phenomenon of bacterial resistance. Journal of Medical Microbiology, v. 58, n. 9, p.1133-1148, 2009.
TRENTIN, D. S. et al. Potential of medicinal plants from the Brazilian semiarid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles. Journal of Ethnopharmacology, v. 137, n. 1, p. 327-335, 2011.
VANDEBROEK, I. et al. Local knowledge: who cares? Journal of Ethnobiology and Ethnomedicine, v. 7, n. 35, p. 1-7, 2011.
WAGNER, H.; BLADT, S. Plant drug analysis: a thin layer chromatography atlas. 2. ed. Berlin: Springer-Verlag, 1996, 369p.
XIAO, M. Antimicrobial susceptibility of Pseudomonas aeruginosa in China: a review of two multicentre surveillance programmes, and application of revised CLSI susceptibility breakpoints. International Journal of Antimicrobial Agents, v. 40, n. 5, p. 445-449, 2012.
ALBUQUERQUE, U. P. et al. Medicinal plants of the Caatinga (semi-arid) vegetation of NE Brazil: a quantitative approach. Journal of Ethnopharmacology, v. 114, n. 3, p. 325-354, 2007a.
ALBUQUERQUE, U. P. et al. Medicinal and magic plants from a public market in northeastern Brazil. Journal of Ethnopharmacology, v. 110, n. 1, p. 76-91, 2007b.
ANDRADE-LIMA, D. The caatinga dominium. Revista Brasileira de Botânica, São Paulo, v. 4, n. 1, p. 149-163, 1981.
ALBUQUERQUE, U. P.; RAMOS, M. A.; MELO, J. G. New strategies for drug Discovery in tropical forests based on ethnobotanical and chemical ecological studies. Journal of Ethnopharmacology, v. 140, n. 1, p. 197-201, 2012.
ARAÚJO, T. A. S. et al. A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge. Journal of Ethnopharmacology, v. 120, n. 1, p. 72-80, 2012.
CARTAXO, S. L.; SOUZA, M. M. A.; ALBUQUERQUE, U. P. Medicinal plants with bioprospecting potential used in semi-arid northeastern Brazil. Journal of Ethnopharmacology, v. 131, n. 2, p. 326-342, 2010.
CEGELSKI, L. et al. The biology and future prospects of antivirulence therapies. Nature Reviews Microbiology, v. 6, n. 11, p. 17-27, 2008.
DING, X. et al. Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm. Journal of Medical Microbiology, v. 60, n. 12, p. 1827-1834, 2011.
GORLACH-LIRA, K.; COUTINHO, H. D. M. Population dynamics and extracellular enzymes activity of mesophilic and thermophilic bacteria isolated from semi-arid soil of Northeastern Brazil. Brazilian Journal of Microbiology, São Paulo, v. 38, n. 1, p. 135-141, 2007.
HERRERA, R. M. et al. Antimicrobial activity of extracts from plants endemic to the Canary Islands. Phytotherapy Research, v. 10, n. 4, p. 364-66, 1996.
KOKOSKA, L. et al. Screening of some Siberian medicinal plants for antimicrobial activity. Journal of Ethnopharmacology, v. 82, n. 1, p. 51-53, 2002.
KONÉ, W. M. et al. Traditional medicine in north Côte-d'Ivoire: screening of 50 medicinal plants for antibacterial activity. Journal of Ethnopharmacology, v. 93, n. 1, p. 43-49, 2004.
KUMAR, V. P. et al. Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology, v. 107, n. 2, p. 182-188, 2006.
LOPEZ, A.; HUDSON, J. B.; TOWERS, G. H. Antiviral and antimicrobial activities of Colombian medicinal plants. Journal of Ethnopharmacology, v. 77, n. 2-3, p. 189-196, 2001.
MARTÍNEZ, M. J. et al. Screening of some Cuban medicinal plants for antimicrobial activity. Journal of Ethnopharmacology, v. 52, n. 3, p. 171-74, 1996.
MEDEIROS, M. F.; ALBUQUERQUE, U. P. The pharmacy of the Benedictine monks: the use of medicinal plants in Northeast Brazil during the nineteenth century (1823-1829). Journal of Ethnopharmacology, v. 139, n. 1, p. 280-286, 2012.
MONTEIRO, J. M. et al. Dynamics of medicinal plants knowledge and commerce in an urban ecosystem (Pernambuco, Northeast Brazil). Environmental Monitoring and Assessment, v. 178, n. 1-4, p. 179-202, 2011.
MOTHANA, R. A.; LINDEQUIST, U. Antimicrobial activity of some medicinal plants of the island Soqotra. Journal of Ethnopharmacology, v. 96, n. 1-2, p. 177-181, 2005.
ORTEGA, F. Medicinal plants in the evolution of therapeutics - a case of applied ethnopharmacology. In: ELISABETSKY, E.; ETKIN, N.L. (Eds.). Ethnopharmacology. Oxford, UK: Eolss Publishers, 2006, p.160–184.
RICHARDS, M. J. et al. Nosocomial infections in medical intensive care units in the United States. National Nosocomial Infections Surveillance System. Critical Care Medicine, v. 27, n. 5, p. 887-892, 1999.
RYBTKE, M. T. et al. The implication of Pseudomonas aeruginosa biofilms in infections. Inflammation & Allergy-Drug Targets, v. 10, n. 2, p. 141-157, 2011.
ROSSI, F. The challenges of antimicrobial resistance in Brazil. Clinical Infectious Disease, v. 52, n. 9, p. 1138-1143, 2011.
SAMPAIO, E. V. S. B. et al. Vegetação e flora da caatinga. Recife: Associação Plantas do Nordeste, 2002. p.176.
SIQUEIRA, C. F. et al. Levels of tannins and flavonoids in medicinal plants: evaluating bioprospecting strategies. Evidence-Based Complementary and Alternative Medicine, Article ID 434782, 2012.
SOULI, M.; GALANI, I.; GIAMARELLOU, H. Emergence of extensively drug-resistant and pandrug-resistant Gram-negative bacilli in Europe. Euro Surveillance, v. 13, n. 47, p. 1-11, 2008.
STRATEVA, T.; YORDANOV, D. Pseudomonas aeruginosa - a phenomenon of bacterial resistance. Journal of Medical Microbiology, v. 58, n. 9, p.1133-1148, 2009.
TRENTIN, D. S. et al. Potential of medicinal plants from the Brazilian semiarid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles. Journal of Ethnopharmacology, v. 137, n. 1, p. 327-335, 2011.
VANDEBROEK, I. et al. Local knowledge: who cares? Journal of Ethnobiology and Ethnomedicine, v. 7, n. 35, p. 1-7, 2011.
WAGNER, H.; BLADT, S. Plant drug analysis: a thin layer chromatography atlas. 2. ed. Berlin: Springer-Verlag, 1996, 369p.
XIAO, M. Antimicrobial susceptibility of Pseudomonas aeruginosa in China: a review of two multicentre surveillance programmes, and application of revised CLSI susceptibility breakpoints. International Journal of Antimicrobial Agents, v. 40, n. 5, p. 445-449, 2012.
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26-09-2014
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