Micropropagation of Vellozia pyrantha A. A. Conc., an endemic species from Chapada Diamantina
DOI:
https://doi.org/10.1590/1983-21252026v3913704rcKeywords:
Candombá. Velloziaceae. In vitro culture. Shoots. Sealing.Abstract
Chapada Diamantina is mainly composed of campos rupestres (rupestrian grasslands), harboring endemic plants that contribute to the ornamentation of this landscape, such as species of the genus Vellozia. Vellozia pyrantha is commonly known as “candombá” and has cultural and economic importance to the local people. These plants exhibit unusual phenological aspects, due to their dependence on fire to flower, being able to regenerate after being burned. Given the phenological uniqueness of candombá, associated with the destruction arising from extractive exploitation and the lack of studies about its propagation, this study was conceived aiming to establish a micropropagation protocol for the species. In vitro establishment was carried out using disinfected seeds, inoculated in MS ⅓ medium, supplemented with 15 g L-1 of sucrose and 7 g L-1 of agar; the plantlets were transferred for in vitro multiplication, in a medium containing BAP (0.00; 4.44; 8.88 and 17.76 µM). The obtained shoots were subjected to in vitro rooting, testing the addition of activated charcoal to the medium and two types of container sealing (PVC or cotton plug). Rooted microplants were subjected to acclimatization in a greenhouse. About 9,35 shoots were obtained when inducing multiplication with 8.88 µM of BAP. In the rooting stage, charcoal favored the lengths of the aerial and root parts, whereas the use of cotton plug favored the number and length of roots. Acclimatized plants achieved a survival rate between 80 and 95% after 30 days of acclimatization, implying that micropropagation is an efficient technique to produce seedlings of V. pyrantha.
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