IDENTIFICATION AND EFFECTS OF MIXED INFECTION OF Potyvirus ISOLATES WITH Cucumber mosaic virus IN CUCURBITS

Authors

  • Graziela da Silva Barbosa Plant Molecular Biology Laboratory, Technology and Social Sciences Department, Universidade do Estado da Bahia, Juazeiro, BA
  • José Albersio de Araújo Lima Plant Virology Laboratory, Department of Phytosanitation, Universidade Federal do Ceará, Fortaleza, CE
  • Manoel Abilio Queiróz Plant Molecular Biology Laboratory, Technology and Social Sciences Department, Universidade do Estado da Bahia, Juazeiro, BA
  • Rita de Cássia Souza Dias Embrapa Semiárido, Petrolina, PE
  • Cristiano Souza Lima Plant Virology Laboratory, Department of Phytosanitation, Universidade Federal do Ceará, Fortaleza, CE

DOI:

https://doi.org/10.1590/1983-21252016v29n429rc

Keywords:

Papaya ringspot virus. Zucchini yellow mosaic virus. Watermelon mosaic virus. Synergistic interaction.

Abstract

Mixed infections in cucurbits are frequently observed in natural conditions between viruses from the Potyvirus genus and Cucumber mosaic virus (CMV), which significantly decreases productivity. The objectives of the present study was to compare the host range of PRSV-W, WMV, and ZYMV isolates and evaluate the effects of mixed infections with CMV in zucchini plants (Cucurbita pepo L.). Host range studies comprising 23 plant species confirmed some similarities and biological differences among the isolates of PRSV-W, ZYMV, and WMV. RT-PCR confirmed the amplification of DNA fragments of the PRSV-W, WMV, and ZYMV coat protein gene (cp) and cytoplasm inclusion gene (ci). The virus interaction studies in zucchini Caserta plants indicated synergistic interactions, particularly among species from the Potyvirus genus, and some CMV interference with some virus combinations.

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Published

13-09-2016

Issue

Section

Technical Note