Aerobiological and molecular monitoring of banana sigatoka pathogens and strobilurin resistance

Authors

  • Abimael Gomes da Silva Department of Crop Protection, Agricultural Engineering and Soil, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Ilha solteira, SP, Brazil https://orcid.org/0000-0001-7146-9226
  • Loane Dantas Krug Department of Crop Protection, Agricultural Engineering and Soil, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Ilha solteira, SP, Brazil https://orcid.org/0000-0003-3051-3897
  • Tatiane Carla Silva Faculty of Pharmaceutical Sciences of Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil https://orcid.org/0000-0002-5317-9388
  • Silvino Intra Moreira Institute of Agricultural Sciences, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil https://orcid.org/0000-0003-4693-8806
  • Daniel Macedo de Souza Department of Crop Protection, Agricultural Engineering and Soil, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Ilha solteira, SP, Brazil https://orcid.org/0009-0004-6182-1977
  • Gabriela Valério Leardine Department of Crop Protection, Agricultural Engineering and Soil, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Ilha solteira, SP, Brazil https://orcid.org/0009-0007-9242-5370
  • Maria Cândida de Godoy Gasparoto Universidade Estadual Paulista “Júlio de Mesquita Filho”, Registro, SP, Brazil https://orcid.org/0000-0001-5868-9670
  • Paulo Cezar Ceresini Department of Crop Protection, Agricultural Engineering and Soil, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Ilha solteira, SP, Brazil https://orcid.org/0000-0003-2381-2792 (unauthenticated)

DOI:

https://doi.org/10.1590/1983-21252025v3812568rc

Keywords:

Banana leaf spot diseases. Fungicide resistance. Sigatoka disease complex. Aerobiology monitoring. Disease management innovation.

Abstract

The chemical control of Black and Yellow Sigatoka leaf diseases on bananas, respectively caused by Mycosphaerella fijiensis (Mf) and M. musicola (Mm), is relevant due to the high yield losses reported in the most susceptible plant varieties and to support more sustainable agricultural practices. However, intensive spraying of fungicides has led to the development of resistance and consequent loss of efficacy, demanding new management strategies. This study tested the application of an automated system for airborne spore sampling coupled with real-time quantitative PCR (RT-qPCR) to monitor the aerobiology of Mf and Mm and to detect mutations associated with resistance to QoI (external quinone inhibitors or strobilurins) fungicides in two banana-producing regions in São Paulo State. Total fungal DNA was extracted from the airborne spore samples and subsequently analyzed by qPCR to quantify the fluctuation in the prevalence of Mf and Mm along the year. Specific probes designed for the target-gene cytB were used to detect the prevalence of the fungicide resistance-allele associated with QoI resistance in the spore samples. The RT-qPCR assay accurately detected resistant Mf and Mm present in the aerobiology samples. This molecular approach can guide the choice and the optimal timing for fungicide spraying. Furthermore, the developed technology represents a significant advancement in disease management as agricultural innovation.

References

AFFONSO, V. et al. Analysis of the maximum precipitation data in northwestern São Paulo by theory of extreme values. Research, Society and Development, 9: e9709109396, 2020.

AMORIM, L. et al. Manual de Fitopatologia: Doenças das plantas cultivadas. 5. ed. São Paulo, SP: Agronômica Ceres, 2016. 772 p.

ARZANLOU, M.; CROUS, P. W.; ZWIERS, L.-H. Evolutionary dynamics of mating-type loci of Mycosphaerella spp. occurring on banana. Eukaryotic Cell, 9: 164-172, 2010.

BIRLA, K et al. Characterization of cytochrome b from European field isolates of Cercospora beticola with quinone outside inhibitor resistance. European Journal of Plant Pathology, 134: 475-488, 2012.

BRITO, F. S. D. et al. Sigatoka disease complex of banana in Brazil: Management practices and future directions. Outlooks on Pest Management, 26: 78-81, 2015.

BRITO, F. S. D. et al. Genetic diversity and azole fungicide sensitivity in Pseudocercospora musae field populations in Brazil. Frontiers in Microbiology, 11: 99, 2020.

CERESINI, P. C. et al. Strategies for managing fungicide resistance in the Brazilian tropical agroecosystem: Safeguarding food safety, health, and the environmental quality Tropical Plant Pathology, 49: 1-35, 2024.

CHERRAD, S. et al. New insights from short and long reads sequencing to explore cytochrome b variants in Plasmopara viticola populations collected from vineyards and related to resistance to complex III inhibitors. PLoS One, 19: e0268385, 2023.

CHURCHILL, A. C. L. Mycosphaerella fjiensis, the black leaf streak pathogen of banana: progress towards understanding pathogen biology and detection, disease development, and the challenges of control. Molecular Plant Pathology, 12: 307-328, 2011.

EMILIANO, V. M. Variabilidade espacial e temporal das precipitações pluviais no Vale do Ribeira de Iguape. 2022. 70 f. Trabalho de Conclusão de Curso (Graduação) – Faculdade de Filosofia, Letras e Ciências Humanas, Universidade de São Paulo, São Paulo, 2022.

MANZO-SÁNCHEZ, G. et al. Genetic variability of Pseudocercospora fijiensis, the black Sigatoka pathogen of banana (Musa spp.) in Mexico. Plant Pathology, 68: 513-522, 2019.

MENDONZA, M. J. C.; ARDALES, E. Y. Population structure of the banana black Sigatoka pathogen [Pseudocercospora fijiensis (M. Morelet) Deighton] in Luzon, Philippines. Philippines Agricultural Scientist, 102: 211-219, 2019.

OLIVEIRA, T. Y. K. et al. Evidence of Resistance to QoI Fungicides in Contemporary Populations of Mycosphaerella fijiensis, M. musicola and M. thailandica from Banana Plantations in Southeastern Brazil. Agronomy, 12: 2952, 2022.

R DEVELOPMENT CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing. Vienna: R Foundation for Statistical Computing. 2022. Available at: <http://www.r-project.org/index.html>. Access on: Sept. 25, 2023.

SILVA, T. C. et al. Resistance to site-specific succinate dehydrogenase inhibitor fungicides is pervasive in populations of black and yellow Sigatoka pathogens in banana plantations from Southeastern Brazil. Agronomy, 14: 666, 2024.

UCHÔA, C. D. N. et al. Modelling black Sigatoka epidemics with seasonal dispersal of Mycosphaerella fijiensis ascospores over a banana plantation in the Ribeira Valley, São Paulo, Brazil. European Journal of Plant Pathology, 161: 463-474, 2021.

VICENTINI, S.N.C. et al. Aerobiology of the wheat blast pathogen: inoculum monitoring and detection of fungicide resistance alleles. Agronomy, 13: 1238, 2023.

VIELBA-FERNÁNDEZ, A. et al. Heteroplasmy for the cytochrome b gene in Podosphaera xanthii and its role in resistance to QoI fungicides in Spain. Plant Disease, 102: 1599-1605, 2018.

Downloads

Published

04-11-2025

Issue

Vol. 38 (2025)

Section

Scientific Article

License

Os Autores que publicam na Revista Caatinga concordam com os seguintes termos:

 

a)      Os Autores mantêm os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons  do tipo atribuição CC-BY, para todo o conteúdo do periódico, exceto onde estiver identificado, que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista, sem fins comerciais.

 b)     Os  Autores têm autorização para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.

 c)     Os  Autores têm permissão e são estimulados a publicar e distribuir seu trabalho online (ex.: em repositórios institucionais ou na sua página pessoal) a qualquer ponto antes ou durante o processo editorial, já que isso pode gerar alterações produtivas, bem como aumentar o impacto e a citação do trabalho publicado (Veja O Efeito do Acesso Livre).