DESINFESTATION IN VITRO OF BANANA ‘FARTA VELHACO’ (SUB GROUP AAB)' DIFERENTS CONCENTRATIONS ATIVE CHORO

Authors

  • Gustavo Alves Pereira Centro Regional de Pesquisa e Transferência de Tecnologia (EMPAER-MT), Rua Senador Jonas Pinheiro S/N, Ponte Nova, CEP: 78115-100, Várzea Grande (MT).
  • Marcilio Bobroff Santaella Centro Regional de Pesquisa e Transferência de Tecnologia (EMPAER-MT), Rua Senador Jonas Pinheiro S/N, Ponte Nova, CEP: 78115-100, Várzea Grande (MT)
  • Lefayete Michele Santana Montenegro Alves Centro Regional de Pesquisa e Transferência de Tecnologia (EMPAER-MT), Rua Senador Jonas Pinheiro S/N, Ponte Nova, CEP: 78115-100, Várzea Grande (MT)
  • Elder Cassimiro da Silva Centro Regional de Pesquisa e Transferência de Tecnologia (EMPAER-MT), Rua Senador Jonas Pinheiro S/N, Ponte Nova, CEP: 78115-100, Várzea Grande (MT)

DOI:

https://doi.org/10.1590/1983-21252015v28n407rc

Keywords:

Musa sp. Micropropagation. Desinfestation.

Abstract

Most banana plantations are still made using traditional clones per type horn and rhizomes. Other propagation methods such as micropropagation have been developed and perfected to raise the rate of multiplication in a short time and improve the health status of the seedlings. However, contamination by bacteria and fungi is one of the biggest problems of this technique. The aim was to evaluate the in vitro disinfestation using active chlorine concentrations of banana explants ‘Farta Velhaco’. The experimental design used was completely randomized consisting of five treatments and five repetition, each replicate represented by five explants in different concentrations of active chlorine for twenty minutes: T1 - without active chlorine, T2 - 0.5% active chlorine, T3 - 1.0% of active chlorine, T4 - 1.5% active chlorine and T5 - 2% active chlorine. The percentages of contamination by bacteria and fungi as well as the percentage of oxidation of the explants were evaluated. Data were subjected to analysis of variance and regression analysis. The results showed that the highest efficiency among the tested treatments was the immersion of explants in 1% of active chlorine that caused a reduction in 95% and 90% respectively for bacteria and fungi and 60% of explants oxidation. It was concluded that this concentration can be used to control contamination for micropropagation of banana 'Farta Velhaco'.

Author Biography

  • Gustavo Alves Pereira, Centro Regional de Pesquisa e Transferência de Tecnologia (EMPAER-MT), Rua Senador Jonas Pinheiro S/N, Ponte Nova, CEP: 78115-100, Várzea Grande (MT).
    Engenheiro agronomo fotmado pela UNESP de Ilha SOlteira, mestrado pela ESALQ, doutorado e pós doc pela UNESP de Ilha SOlteira. Bolsista DCR/CNQPQ Na EMPAER-MT com o projeto intitulado Protocolo para micropropagação da bananeira Farta Velhaco.

Published

18-11-2015

Issue

Section

Agronomy