METHODS FOR INOCULATION WITH Fusarium guttiforme AND GENETIC RESISTANCE OF PINEAPPLE (Ananas comosus var. comosus)

Authors

  • Wandreilla Moreira Garcia Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT
  • Willian krause Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT
  • Dejânia Vieira de Araújo Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT
  • Isane Vera Karsburg Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT
  • Rivanildo Dallacort Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT

DOI:

https://doi.org/10.1590/1983-21252017v30n210rc

Keywords:

Ananas comosus var. comosus. Genetic resistance. Fusariosis.

Abstract

The objective of this work was to evaluate Fusarium guttiforme inoculation methods and genetic resistance of pineapple accessions. Thus, three experiments were conducted: pathogen inoculation of different leaf types (B, D and F) of pineapple (1), pathogen inoculation of pineapple cuttings and detached D leaves (2), and identification of resistance to fusariosis in 19 pineapple accessions (3) sampled in the State of Mato Grosso, Brazil. The cultivars Pérola (susceptible to fusariosis) and BRS-Vitória (resistant to fusariosis) were used as controls. The fusariosis severity was evaluated at 10, 15, 20, 25 and 30 days after inoculation with F. guttiforme. The lesion diameters (severity level) were used in order to calculate the area under the disease progress curve (AUDPC). The inoculation of detached D leaves was the most efficient, fast and inexpensive method, and the one that most satisfactorily reproduced the disease symptoms. The period of 10 to 20 days after inoculation of the D detached leaves with the pathogen is the most suitable to evaluate the resistance of pineapple accessions to fusariosis. The lowest lesion area and AUDPC was found in the accession 1, in all evaluations. Thus, the accession 1 can be used in pineapple breeding programs for resistance to fusariosis.

Author Biography

  • Wandreilla Moreira Garcia, Department of Agronomy, Universidade do Estado de Mato Grosso, Tangará da Serra, MT
    Bióloga, Doutoranda em Genética em Melhoramento de Plantas (UENF), Mestre em Genética e Melhoramento de Plantas (UNEMAT-2013) e Especialista em Gestão Ambiental (FIV-2012)

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Published

24-01-2017

Issue

Section

Agronomy