MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL

Authors

  • Elaine Cristina Batista Ferreira Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE
  • Moises Thiago de Souza Freitas Department of Genetic, Universidade Federal de Pernambuco, Recife, PE
  • Karla Diana da Silva Sombra Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE
  • Herbert Álvaro Abreu de Siqueira Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE
  • Elton Lucio de Araujo Department of Plant Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN
  • Valdir de Queiroz Balbino Department of Genetic, Universidade Federal de Pernambuco, Recife, PE

DOI:

https://doi.org/10.1590/1983-21252017v30n409rc

Keywords:

Cryptic species. DNA barcoding. Population genetics.

Abstract

In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra- and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil.

Author Biographies

  • Elaine Cristina Batista Ferreira, Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE
    Departamento de Entomologia Agrícola e Biologia de insetos
  • Moises Thiago de Souza Freitas, Department of Genetic, Universidade Federal de Pernambuco, Recife, PE
    Departamento de Genética e Evolução Molecular
  • Karla Diana da Silva Sombra, Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife, PE
    Departamento de Genética e Genética de populações
  • Valdir de Queiroz Balbino, Department of Genetic, Universidade Federal de Pernambuco, Recife, PE
    Departamento de Genética

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Published

14-06-2017

Issue

Section

Agronomy