TOXICITY OF Esenbeckia pumila Pohl (Rutaceae) ON Artemia salina AND Atta sexdens rubropilosa
DOI:
https://doi.org/10.1590/1983-21252019v32n111rcKeywords:
Phytochemical study. Natural botanical products. Insecticidal activity.Abstract
The number of studies on plant compounds with insecticidal activity has increased in recent years, and one of the primary targets of these compounds is leaf-cutter ants, which are considered the most important pests in Brazilian plantations, especially ants of the genus Atta. The objective of this study was to evaluate the toxic activity of the crude extract and fractions of the leaves of Esenbeckia pumila Pohl (Rutaceae) on Artemia salina and Atta sexdens rubropilosa and to perform a phytochemical study of this plant species. The toxicity of the extract and fractions was evaluated by determining the mean lethal concentration (LC50) on A. salina. The insecticidal activity was evaluated by feeding the ants an artificial diet containing the crude extract or fractions, and the results were analyzed using the log-rank test. The substances were isolated by chromatography, and the molecular structure was determined by spectroscopy. In the bioassay with A. salina, the ethanol extract and dichloromethane and ethyl acetate fractions were highly toxic. The analysis of survival curves indicated that the mortality rate of A. sexdens rubropilosa workers fed different fractions was higher than that of ants fed the control diet, especially the ethyl acetate fraction, with a mean survival time of 3 days and cumulative mortality of 100% on day 21 (p<0.05). The phytochemical study of this plant species allowed isolating the flavonoid rutin and a mixture of the triterpenes α-amyrin, β-amyrin, and lupeol. These results suggest the insecticidal potential of E. pumila on Atta sexdens rubropilosa.
References
ALBUQUERQUE, L. P. et al. Toxic effects of Microgramma vacciniifolia rhizome lectin on Artemia salina, human cells, and the schistosomiasis vector Biomphalaria glabrata. Acta Tropica, v. 138, s/n, p. 23-27, 2014.
ALMEIDA, R. N. A. et al. Toxicity of substances isolated from Helietta puberula RE Fr. (Rutaceae) to the leaf-cutting ant Atta sexdens L. (Hymenoptera: Formicidae) and the symbiotic fungus Leucoagaricus gongylophorus (Singer) Möller. BioAssay, v. 2, n. 2, p. 1-8, 2007.
APU, A. S. et al. Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica Linn. Journal of Young Pharmacists, v. 2, n. 1, p. 50-53, 2010.
BARBOSA, F. R.; SILVA, C. S. B.; CARVALHO, G. K. L. Uso de inseticidas alternativos no controle de pragas agrícolas. Petrolina, PE: EMBRAPA Semi-Árido, 2006. 47 p. (Embrapa Semi-Árido. Documentos, 191).
BIAVATTI, M. W. et al. Leaf-cutting ants toxicity of limonexic acid and degraded limonoids from Raulinoa echinata. X-Ray Structure of Epoxy-fraxinellone. Journal of the Brazilian Chemical Society, v. 16, n. 6, p. 1443-1447, 2005.
BITCHAGNO, G. T. M. et al. Lemairones A and B: Two new antibacterial tetraflavonoids from the leaves of Zanthoxylum lemairei (Rutaceae). Phytochemistry Letters, v. 14, s/n, p. 1-7, 2015.
BUENO, O. C. et al. Sobrevivência de operárias de Atta sexdens rubropilosa Forel (Hymenoptera: Formicidae) isoladas do formigueiro e alimentadas com dietas artificiais. Anais da Sociedade Entomológica do Brasil, v. 26, n. 1, p. 107-113, 1997.
CAMPOS, S. C. et al. Toxicidade de espécies vegetais. Revista Brasileira Plantas Medicinais, v. 18, n. 1, Sup., p. 373-382, 2016.
CANTARELLI, E. B. et al; Efeito de diferentes doses do formicida “Citromax” no controle de Acromyrmex lundi (Hymenoptera: Formicidae). Ciência Florestal, v. 15, n. 3, p. 249-253, 2005.
CARDOSO-LOPES, E. M. et al. Alkaloids from stems of Esenbeckia leiocarpa Engl. (Rutaceae) as potential treatment for Alzheimer Disease. Molecules, v. 15, n. 12, p. 9205-9213, 2010.
COY-BARRERA, C. A.; CUCA-SUÁREZ, L. E.; LONDOÑO, C. Q. Revista Cubana de Plantas Medicinales, v. 18, n. 4, p. 638-653, 2013.
CUCA-SUÁREZ, L. E et al Actividad antibacteriana de terpenoides y alcaloides aislados de tres plantas colombianas. Revista Cubana de Farmacia, v. 45, n. 2, p. 275-282, 2011.
DELLA LUCIA, T. M. C.; GANDRA, L. C.; GUEDES, R. N. C. Managing leaf-cutting ants: peculiarities, trends and challenges. Pest Management Science, v. 70, n. 1, p. 14-23, 2014.
DOLABELA, M. F. et al. In vitro antiplasmodial activity of extract and constituents from Esenbeckia febrifuga, a plant traditionally used to treat malária in the Brazilian Amazon. Phytomedicine, v. 15, n. 5, p. 367-372, 2008.
ELANDT–JOHNSON, R. C.; JOHNSON, N. L. Survival models and data analysis. Toronto: Wiley-Interscience, 1980. 457 p.
FERNANDES, J. B. et al. Extrações de óleos de sementes de citros e suas atividades sobre a formiga cortadeira Atta sexdens e seu fungo simbionte. Química Nova, v. 25, n. 6, p. 1091-1095, 2002.
GOMES, M. C. A. R. et al. Toxicity of plant extracts from Bahia, Brazil, to Atta sexdens (Hymenoptera: Formicidae) workers. Sociobiology, v. 63, n. 2, p. 770-776, 2016.
GUARDA, C. et al. Atividade larvicida de produtos naturais e avaliação da susceptibilidade ao inseticida Temefós no controle do Aedes aegypti (Diptera: Culicidae). Interciência, v. 41, n. 4, p. 243-247, 2016.
KUBO, I. Recent applications of counter-current chromatography the isolation of bioactive natural products. Journal of Chromatography, v. 538, n. 1, 187-191, 1991.
KUBO, I.; VIEIRA, P. C.; FUKUHARA, K. Efficient isolation of the insect growth inhibitory flavone glycoside rutin from two tropical medicinal plants by Rotation Locular Countercurrent Chromatography (RLCC). Journal of Liquid Chromatography, v. 13, n. 12, p. 2441-2448, 1990.
KUETE, V. et al. Antimicrobial activity of the methanolic extract and compounds from Teclea afzelii (Rutaceae). South African Journal of Botany, v. 74, n. 4, p. 572-576, 2008.
LEITE, J. J. G. et al. Chemical composition, toxicity and larvicidal and antifungal activities of Persea americana (avocado) seed extracts. Revista da Sociedade Brasileira de Medicina Tropical, v. 42, n. 2, p. 110-113, 2009.
MATOS, F. J. A. Introdução à fitoquímica experimental. 3. ed. Fortaleza, CE: UFC, 2009. 150 p.
MEYER, B. N. et al. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Medica, v. 45, n. 5, p. 31-34, 1982.
MOLINA-SALINAS, G. M.; SAID-FERNÁNDEZ, S. A modified microplate cytotoxicity assay with brine shrimp larvae (Artemia salina). Pharmacologyonline, v. 3, s/n, p. 633-638, 2006.
MUGANGA, R. et al. In vitro and in vivo antiplasmodial activity of three Rwandan medicinal plants and identification of their active compounds. Planta Medica, v. 80, n. 6, p. 482-489, 2014.
MUKANDIWA, L.; ELOFF, J. N.; NAIDOO, V. Larvicidal activity of leaf extracts and seselin from Clausena anisata (Rutaceae) against Aedes aegypti. South African Journal of Botany, v. 100, s/n, p. 169-173, 2015.
NAPOLITANO, H. B. et al. Aurapten, a coumarin with growth inhibition against Leishmania major promastigotes. Brazilian Journal of Medical and Biological Research, v. 37, n. 12, p. 1847-1852, 2004.
NGUTA, J. M. et al. Biological screening of Kenya medicinal plants using Artemia salina L. (Artemiidae). Pharmacologyonline, v. 2, s/n, p. 458-478, 2011.
NISAR, M. et al. H. Larvicidal, insecticidal, brine shrimp cytotoxicity and anti-oxidant activities of Diospyros kaki (L.) reported from Pakistan. Pakistan Journal of Pharmaceutical Sciences, v. 28, n. 4, p. 1239-1243, 2015.
SILVA, T. R. F. B. et al. Effect of the flavonoid rutin on the biology of Spodoptera frugiperda (Lepidoptera: Noctuidae). Acta Scientiarum. Agronomy, v. 38, n. 2, p. 165-170, 2016.
TEREZAN, A. P. et al. Activities of extracts and compounds from Spiranthera odoratissima St. Hil. (Rutaceae) in leaf-cutting ants and their symbiotic fungus. Journal of the Brazilian Chemical Society, v. 21, n. 5, p. 882-886, 2010.
ZANETTI, R. et al. An overview of integrated management of leaf-cutting ants (Hymenoptera: Formicidae) in brazilian forest plantations. Forests, v. 5, n. 3, p. 439-454, 2014.
Downloads
Published
Issue
Section
License
Os Autores que publicam na Revista Caatinga concordam com os seguintes termos:
a) Os Autores mantêm os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons do tipo atribuição CC-BY, para todo o conteúdo do periódico, exceto onde estiver identificado, que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista, sem fins comerciais.
b) Os Autores têm autorização para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.
c) Os Autores têm permissão e são estimulados a publicar e distribuir seu trabalho online (ex.: em repositórios institucionais ou na sua página pessoal) a qualquer ponto antes ou durante o processo editorial, já que isso pode gerar alterações produtivas, bem como aumentar o impacto e a citação do trabalho publicado (Veja O Efeito do Acesso Livre).
