Antiparasitic action in vitro of ethanolical extracts of Operculina hamiltonii (“Batata de Purga”) and Momordica charantia (“Melão de São Caetano”) on eggs and larvae of gastrintestinal nematodes of goats from the semiarid of Paraiba, Brazil

Abstract

The objective of this work was to evaluate in vitro the action of the ethanolical extracts of Operculina hamiltonii (G. DON) D. F. Austin & Staples (1983) - Batata de purga and Momordica charantia Linnaeus (1763) - Melão de São Caetano on eggs and larvae of gastrintestinal nematodes of goats. Making the ethanolical extracts and the phytochemical study followed the methodology described by Matos (1997) in which it the flour of the recommended shares of each species was used. Four tests were performed for the prospect of constituents of the hidralcoholic extract. The recovery of eggs was done in tamises and the larvae were obtained through farming larvae from faeces of naturally infected goats from the semiarid of Paraíba, Brazil. The extract was used in the concentrations 50; 25; 12; 6 and 3 mg.mL-1 for all plants and as positive control 0,2 mg.kg1 of albendazole 5% and sterile distilled water was used to control. The plates were examined under an optical microscope to count developing eggs and moving and moveless larvae after 24h, 48h and 72h of incubation. It was concluded that the ethanolical extracts of Batata de purga and Melão de São Caetano represent a viable alternative for the control of parasites, demonstrating anthelmintic effectiveness for gastrintestinal nematodes of goats. Keywords: anthelmintic, goats, botanical extracts