Effects of oxygen supplementation in ewes subjected to laparoscopic ova collection under total intravenous anesthesia

Résumé

Laparoscopy procedures are useful for the application of some assisted reproductive biotechnologies in ewes, it requires general anaesthesia and manoeuvres that might result in cardiopulmonary function alterations. For this reason, this study aimed to investigate the effects of oxygen supplementation as a therapeutic measure to mitigate these alterations in ewes submitted to laparoscopic ova collection (LOC) under total intravenous anaesthesia (TIVA). Twenty-four healthy adult ewes were submitted to two LOCs 21 days interval, under ketamine-midazolam anaesthesia, receiving each of the two experimental in random order, oxygen treatment (OT) 50 mL/kg/min of oxygen via endotracheal tube and control treatment (CT) not receive any inhalation treatment. Heart (HR), respiratory rate (f_R), peripheral oxygen saturation (SpO₂), mean arterial pressure (MAP), rectal temperature (RT), end-tidal CO₂ concentration (EtCO₂) and recovery anaesthesia time were evaluated during LOC, arterial blood gases and electrolytes were evaluated after anaesthesia induction and at the LOC end. Variables were compared between groups and moments using ANOVA. MAP, SpO₂, PaO2, SaO2 and pH were higher in OT, while EtCO₂, PaCO2, temperature loss and recovery time were lower. These results allow to conclude that oxygen supplementation in ewes submitted to LOC under TIVA provides benefits in order to mitigate physiological alterations.