MODIFIED TOXIN-BINDING INHIBITION (TOBI) TEST FOR QUANTIFICATION OF EPSILON ANTITOXIN IN SERUM FROM IMMUNIZED SHEEP

Autori

  • Eliane Macedo Sobrinho Postgraduate (MSc) Student in Agricultural Sciences - Instituto de Ciências Agrárias, UFMG and teacher in the Instituto Federal do Norte de Minas Gerais, IFNMG, Araçuaí, MG
  • Anna Christina Almeida UFMG
  • Hercules Otacílio Santos USP
  • Alex Sander Rodrigues Cangussu Vallée
  • Fernando Colen UFMG
  • Igor Viana Brandi UFMG
  • Roger Soger Sari Vallee
  • Wagner Quintilio Instituto Butantan

DOI:

https://doi.org/10.21708/avb.2011.5.3.2328

Abstract

This study evaluated the use of the ToBI test as a substitute for the in vivo technique of the mouse serum neutralization test to manage quality control of vaccines against clostridiosis. Serum samples with known concentrations of anti-epsilon antibodies were obtained from 3 sheep immunized with vaccine against C. perfringens type D epsilon toxoid. The main results obtained indicate correlation coefficients above 97%, corroborating literature data. The inter and intraplate coefficients of variation were low (less than 1.3%). In conclusion, the in vitro ToBI test is suitable to evaluate the potency of clostridial vaccines. However, the ToBI test must be first validated before it can replace the in vivo serum neutralization methodology used in mice. Keywords: Clostridiosis, in vitro assay, potency testing.

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Biografie autore

Eliane Macedo Sobrinho, Postgraduate (MSc) Student in Agricultural Sciences - Instituto de Ciências Agrárias, UFMG and teacher in the Instituto Federal do Norte de Minas Gerais, IFNMG, Araçuaí, MG

Professora efetiva das áreas de Agroecologia e Gestão Ambiental

Anna Christina Almeida, UFMG

Professora Efetiva da área de Ciência Animal

Hercules Otacílio Santos, USP

Mestrando em Biotecnologia

Alex Sander Rodrigues Cangussu, Vallée

Supervisor de Produção Biológica

Fernando Colen, UFMG

Professor da área de Bioenergia

Igor Viana Brandi, UFMG

Professor efetivo de engenharia de alimentos

Roger Soger Sari, Vallee

Supervisor de Produção Biológica

Wagner Quintilio, Instituto Butantan

Controle de Qualidade

Pubblicato

2012-01-27

Fascicolo

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Original Articles / Artigos de Pesquisa

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