Follicular survival, activation of primordial follicles and DNA fragmentation after storage of goat ovaries at 35ºC in supplemented Minimal Essential Medium

Autori

  • Rodrigo José Sousa Gonçalves Universidade Federal do Vale do São Francisco
  • Agnes Yasmin Pitombeira Cavalcante Universidade Federal do Vale do São Francisco
  • Bruna Bortoloni Gouveia Universidade Federal do Vale do São Francisco
  • Thae Lanne Barbosa Lins Universidade Federal do Vale do São Francisco
  • Ricássio Sousa Barberino Universidade Federal do Vale do São Francisco
  • Vanúzia Gonçalves Menezes Universidade Federal do Vale do São Francisco
  • Vanessa Raquel Pinto Barros Universidade Federal do Vale do São Francisco
  • Luciana Paz Santos Universidade Federal do Vale do São Francisco
  • Jamile Maiara Silva Santos Universidade Federal do Vale do São Francisco
  • José Ricardo Figueiredo Universidade Estadual do Ceará
  • Maria Helena Tavares Matos Universidade Federal do Vale do São Francisco

DOI:

https://doi.org/10.21708/avb.2017.11.1.6660

Abstract

This study evaluated the effect of caprine ovarian tissue transportation conditions (medium supplementation and transportation duration) on the morphology, DNA fragmentation and development of cultured and non-cultured preantral follicles. After the fragmentation of ovaries, one fragment was fixed (fresh control) while the remaining slices were placed individually in two different conservation media (Minimal Essential Medium - MEM without supplementation or supplemented MEM, i.e. MEM+) and stored at 35ºC for 6 or 12 h without (non-cultured) or with a subsequent 5-day in vitro culture in supplemented α-MEM. After transportation, followed or not by in vitro culture, the fragments were processed for histological and Terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) examination. For the preserved and non-cultured fragments, the percentages of normal follicles after the storage of ovarian tissue in MEM+ for 6 h and the DNA fragmentation rates after preservation in MEM for 6 h and MEM+ for 6 or 12 h were maintained similar to the fresh control. However, all cultured treatments reduced the proportion of normal follicles and increased the percentage of TUNEL-positive cells as compared to the fresh control and non-cultured treatments. On the contrary, all culture conditions (except after preservation in MEM for 6 h) promoted an increase in primordial follicle activation. In conclusion, the use of an enriched medium (MEM+) during ovary transportation is preferable to maintain satisfactory rates of normal follicles after the preservation of caprine ovarian tissue at 35ºC for up to 6 h, without affecting the ability of the primordial follicle to grow in vitro.

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Pubblicato

2017-04-18

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Original Articles / Artigos de Pesquisa

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