Screening of bacterial isolates antagonists and suppressors of blast in rice plants

Akintunde Abiodun Ajulo; Rodrigo Silva de  Oliveira; Soraia Ferreira Bezerra; Niedja Bezerra Costa; Ariany Rosa Gonçalves; Maythsulene Inacio de Sousa Oliveira; Marta Cristina Corsi de Filippi

doi:10.1590/1983-21252024v3711724rc

Autores

Akintunde Abiodun Ajulo Postgraduate Program in Agronomy, Universidade Federal de Goiás, Goiânia, GO, Brazil https://orcid.org/0000-0003-1574-5200
Rodrigo Silva de Oliveira Postgraduate Program in Agronomy, Universidade Federal de Goiás, Goiânia, GO, Brazil. https://orcid.org/0000-0003-0015-9401
Soraia Ferreira Bezerra Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0002-4468-4307
Niedja Bezerra Costa Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0002-3914-578X
Ariany Rosa Gonçalves Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0001-6887-5362
Maythsulene Inacio de Sousa Oliveira Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0001-5997-5898
Marta Cristina Corsi de Filippi Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0003-1676-8164

DOI:

https://doi.org/10.1590/1983-21252024v3711724rc

Palavras-chave:

Bioagentes. Biocontrole. Inoculação. Magnaporthe oryzae. Oryza sativa.

Resumo

A produtividade do arroz (Oryza sativa) é afetado mundialmente pela brusone do arroz (Magnaporthe oryzae). O principal objetivo deste estudo foi identificar isolados de rizobactérias antagonistas de M. oryzae (BRM10781) e selecionar os isolados mais eficazes para suprimir a brusone do arroz em condições de casa de vegetação. Foram realizados dois ensaios (E1 e E2) com 22 tratamentos em um delineamento inteiramente casualizado com três repetições. E1 investigou o antagonismo in vitro entre 21 isolados e M. oryzae em condições de laboratório. O experimento E2 foi conduzido em condições de casa de vegetação, com sementes de arroz da cultivar BRS Primavera em bandejas plásticas contendo 3 kg de solo adubado. Após 21 dias, as folhas de arroz foram inoculadas por aspersão com uma suspensão de células bacterianas (1 × 10⁸ UFC) e M. oryzae (3 × 10⁵ conídios.mL^-1) ou com água (controle absoluto). Sete isolados, Serratia marcescens (BRM65918, BRM65923, BRM65926 e BRM63532), Bacillus cereus (BRM65919), Stenotrophomonas nitritireducens (BRM65917) e Priestia megaterium (BRM65929), reduziram o crescimento radial de colônias de M. oryzae de 80,26 para 77,33%. Os melhores supressores da severidade da brusone foliar foram Pseudomonas nitroreducens (BRM32112), B. thuringiensis (BRM65928), P. megaterium (BRM65916), S. marcescens (BRM65918), S. nematodiphila (BRM63522) e Enterobacter hormaechei (BRM65925), variando de 97 a 95%, respectivamente. O isolado BRM65918 (S. marcescens) apresentou a melhor eficiência tanto para o antagonismo quanto para a supressão da doença, indicando seu potencial como bioproduto para o biocontrole da brusone em plantas de arroz.

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