Screening of bacterial isolates antagonists and suppressors of blast in rice plants

Akintunde Abiodun Ajulo; Rodrigo Silva de  Oliveira; Soraia Ferreira Bezerra; Niedja Bezerra Costa; Ariany Rosa Gonçalves; Maythsulene Inacio de Sousa Oliveira; Marta Cristina Corsi de Filippi

doi:10.1590/1983-21252024v3711724rc

Authors

Akintunde Abiodun Ajulo Postgraduate Program in Agronomy, Universidade Federal de Goiás, Goiânia, GO, Brazil https://orcid.org/0000-0003-1574-5200
Rodrigo Silva de Oliveira Postgraduate Program in Agronomy, Universidade Federal de Goiás, Goiânia, GO, Brazil. https://orcid.org/0000-0003-0015-9401
Soraia Ferreira Bezerra Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0002-4468-4307
Niedja Bezerra Costa Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0002-3914-578X
Ariany Rosa Gonçalves Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0001-6887-5362
Maythsulene Inacio de Sousa Oliveira Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0001-5997-5898
Marta Cristina Corsi de Filippi Agricultural Microbiology Laboratory, Embrapa Arroz e Feijão, Santo Antônio de Goiás, GO, Brazil https://orcid.org/0000-0003-1676-8164

DOI:

https://doi.org/10.1590/1983-21252024v3711724rc

Keywords:

Bioagents. Biocontrol. Inoculation. Magnaporthe oryzae. Oryza sativa.

Abstract

Grain yields of rice (Oryza sativa) are affected globally by rice blast (Magnaporthe oryzae). The main objective of this study was to identify isolates of rhizobacterial antagonists of M. oryzae (BRM10781) and screen the most effective isolates for suppressing rice blast under greenhouse conditions. Two assays (E1 and E2) were performed with 22 treatments in a completely randomized design with three replicates. E1 investigated in vitro antagonism between 21 isolates and M. oryzae under laboratory conditions. The E2 experiments were conducted under greenhouse conditions, with rice cultivar BRS Primavera seeds in plastic trays containing 3 kg of fertilized soil. After 21 days, the rice leaves were spray-inoculated with a bacterial cell suspension (1 × 10⁸ CFU) and M. oryzae (3 × 10⁵ conidia.mL^-1) or with water (absolute control). Seven isolates, Serratia marcescens (BRM65918, BRM65923, BRM65926, and BRM63532), Bacillus cereus (BRM65919), Stenotrophomonas nitritireducens (BRM65917), and Priestia megaterium (BRM65929), reduced radial growth of M. oryzae colonies from 80.26 to 77.33%. The best leaf blast severity reducers were Pseudomonas nitroreducens (BRM32112), B. thuringiensis (BRM65928), P. megaterium (BRM65916), S. marcescens (BRM65918), S. nematodiphila (BRM63522), and Enterobacter hormaechei (BRM65925), varying from 97 to 95% respectively. The isolate BRM65918 (S. marcescens) showed the best efficiency for both antagonism and disease suppression, indicating its potential as a bioproduct for the biocontrol of rice blast in rice plants.

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