VERTICAL AND HORIZONTAL RESISTANCE OF F5:6 PROGENIES OF CAROTENOID-BIOFORTIFIED LETTUCE TO Bremia lactucae

Ana Carolina Pires Jacinto; Renata  Castoldi; Gabriel Mascarenhas  Maciel; Jair Rocha do Prado; Hamilton César de Oliveira  Charlo

doi:10.1590/1983-21252022v35n413rc

Authors

Ana Carolina Pires Jacinto Institute of Agricultural Sciences, Universidade Federal de Uberlândia, Uberlândia, MG https://orcid.org/0000-0001-8184-5803
Renata Castoldi Institute of Agricultural Sciences, Universidade Federal de Uberlândia, Monte Carmelo, MG https://orcid.org/0000-0001-9406-0917
Gabriel Mascarenhas Maciel Institute of Agricultural Sciences, Universidade Federal de Uberlândia, Monte Carmelo, MG https://orcid.org/0000-0002-3004-9134
Jair Rocha do Prado Institute of Agricultural Sciences, Universidade Federal de Uberlândia, Monte Carmelo, MG https://orcid.org/0000-0002-6165-4126
Hamilton César de Oliveira Charlo Department of Agronomy, Instituto Federal de Educação, Ciência e Tecnologia do Triângulo Mineiro, Uberaba, MG https://orcid.org/0000-0003-0663-2167

DOI:

https://doi.org/10.1590/1983-21252022v35n413rc

Keywords:

Lactuca sativa (L.). Downy mildew. Non-specific resistance. Specific resistance.

Abstract

The use of resistant cultivars is one of the strategies for downy mildew management. The objective of this study was to evaluate the vertical and horizontal resistance of F_5:6progenies of carotenoid-biofortified lettuce to virulence phenotypes of Bremia lactucae 63/63/51/00, 63/31/19/00 and 63/63/19/00. The experimental design was completely randomized and subdivided into plots that were evaluated over time. In the plots, 12 genotypes of lettuce were used, and the subplots were monitored over time (7^th to the 18^th day after inoculation). For each virulence phenotypes of Bremia lactucae, a separate experiment was performed with three replicates. To select resistant genotypes, plants were inoculated with distilled water, sporangia removed from infected tissues and Tween 20. The genotypes were evaluated when sporulation appeared on cotyledonary leaves of the susceptible cultivar Solaris, verifying the proportion of necrotic and sporulated plants. There was a correlation between genotypes and times for all virulence phenotypes evaluated. Genotypes UFU-189#2, UFU-206#1, UFU-215#3 and UFU-215#14 showed vertical resistance to virulence phenotypes 63/63/51/00, 63/31/19/00, and 63/63/19/00 of B. lactucae. Horizontal resistance levels were observed in genotype UFU 206#1 for virulence phenotypes 63/63/51/00 and 63/31/19/00; in genotype UFU-66#7 for virulent phenotype 63/31/19/00; and in genotype UFU-215#10 for virulence phenotype 63/63/19/00.

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References

ARAÚJO, J. C. et al. Reação de resistência ao míldio e seleção de genótipos nacionais resistentes em população F2 de alface americana. Revista Agrogeoambiental, 6: 11-19, 2014.

BALINT-KURTI, P. The plant hypersensitive response: concepts, control and consequences. Molecular Plant Pathology, 20: 1163-1178, 2019.

BESPALHOK, J. C.; GUERRA, E. P.; OLIVEIRA, R. Noções de genética quantitativa. Disponívem em: http://www.bespa.agrarias.ufpr.br/paginas/livro/capitulo%205. pdf. Acesso em: 17 Jun. 2018.

BILAL, M. et al. High-value compounds from microalgae with industrial exploitability–a review. Frontiers in Bioscience, 9: 319-342, 2017.

CASSETARI, L. S. et al. β-Carotene and chlorophyll levels in cultivars and breeding lines of lettuce. Acta Horticulturae, 1083: 469-474, 2015.

CASTOLDI, R. et al. Identification of new Bremia lactucae races in lettuce in São Paulo state. Horticultura Brasileira, 30: 209-213, 2012.

CASTOLDI, R. et al. Obtaining resistant lettuce progenies to downy mildew. Horticultura Brasileira, 32: 69-73, 2014.

CENTELLA, M. H. et al. Marine-derived bioactive compounds for value-added applications in bio-and non-bio sectors. Journal of Cleaner Production, 168: 1559-1565, 2017.

FALL, M. L. et al. Bremia lactucae infection efficiency in lettuce is modulated by temperature and leaf wetness duration under Quebec field conditions. Plant Disease, 99: 1010–1019, 2015.

FALL, M. L.; VAN DER HEYDEN, H.; CARISSE, O. A quantitative dynamic simulation of Bremia lactucae airborne conidia concentration above a lettuce canopy. Plos One, 11: 1-17, 2016.

FRANCO, C. A. et al. Monitoring virulence of Bremia lactucae as a breeding tool against lettuce downy mildew from south and southwest Brazilian regions. European Journal of Plant Pathology, 159: 179-189, 2021.

ILOTT, T. W.; DURGAN, M. E.; MICHELMORE, R. W. Genetics of virulence in California populations of Bremia lactucae (lettuce downy mildew). Phytopathology, 77: 1381-1386, 1987.

ISF - International Seed Federation. The international Bremia evaluation board (IBEB). Available at:http://www.worldseed.org/isf/ibeb.html. Accessed on: June 27, 2018.

JACINTO, A. C. P. et al. Genetic diversity, agronomic potential and reaction to downy mildew in genotypes of biofortified mini lettuce. Genetics and Molecular Research, 18: 1-10, 2019.

KIMATI, H. et al. Manual de fitopatologia: doenças das plantas cultivadas. 4. ed. São Paulo, SP: Ceres, 2005. 706 p.

MACIEL, G. M. et al. Image phenotyping of inbred red lettuce lines with genetic diversity regarding carotenoid levels. International Journal of Applied Earth Observation and Geoinformation, 81: 154–160, 2019.

MAISONNEUVE, B. Improvement of the differential lettuce set for Bremia virulence evaluation: new sativa monogenic lines. Eucarpia Leafy Vegetables, 61: 24-26, 2011.

MATIELLO, R. R.; BARBIERI, R. L.; CARVALHO, F. I. F. Resistência das plantas a moléstias fúngicas. Ciência Rural, 27: 161-168, 1997.

MICHELMORE, R.; WONG, J. Classical and molecular genetics of Bremia lactucae, cause of lettuce downy mildew. European Journal of Plant Pathology, 122: 19-30, 2008.

MONEGO, D. L.; ROSA, M. B.; NASCIMENTO P. C. Applications of computational chemistry to the study of the antiradical activity of carotenoids: a review. Food Chemistry, 217: 37-44, 2017.

NUNES, R. C. et al. Levantamento de raças do agente causador do míldio da alface no Estado de São Paulo em 2012 e 2013. Summa Phytopathologica, 42: 53-58, 2016.

PARRA, L. et al. Rationalization of genes for resistance to Bremia lactucae in lettuce. Euphytica, 210: 309-326, 2016.

PARRA, L.; SIMKO, I.; MICHELMORE, R. W. Identification of Major Quantitative Trait Loci Controlling Field Resistance to Downy Mildew in Cultivated Lettuce (Lactuca sativa). Phytopathology, 111: 541-547, 2021.

R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, 2016. Available at: http://www.R-project.org/. Accessed on: June 27, 2018.

SIMKO, I. et al. Identification of QTLs conferring resistance to downy mildew in legacy cultivars of lettuce. Scientific Reports, 3: 1-10, 2013.

SOSA-HERNÁNDEZ, J. E. et al. State-of-the-Art Extraction Methodologies for Bioactive Compounds from Algal Biome to Meet Bio-Economy Challenges and Opportunities. Molecules, 23: 2-28, 2018.

SOUSA, L. A. et al. Agronomic potential of biofortified crisphead lettuce (Lactuca sativa) and its reaction to rootknot nematodes. Australian Journal of Crop Science, 13: 773-779, 2019.

STASSEN, J. H. M. et al. Effector identification in the lettuce downy mildew Bremia lactucae by massively parallel transcriptome sequencing. Molecular Plant Pathology, 13: 719-731, 2012.

TOBAR-TOSSE, D. E. et al. Resistance of green leaf lettuce lines to the Bremia lactucae races identified in São Paulo State. Summa Phytopathologica, 43: 55-57, 2017.

ZEILMAKER, T. G. et al. Downy mildew resistant 6 and dmr6-like oxygenase 1 are partially redundant but distinct suppressors of immunity in Arabidopsis. The Plant Journal, 81: 210–222, 2015.