High resistance levels in brazilian Plutella xylostella populations: needs for adjustments in field concentration

José Gomes da Silva Filho; Thiago Izaquiel de Farias; Iuri Andrade de Melo; Kleber Régis  Santoro; Sylvia Anton; César Auguste Badji

doi:10.1590/1983-21252023v36n106rc

Authors

José Gomes da Silva Filho Department of Phytossanity, Universidade Federal de Pelotas, Pelotas, RS https://orcid.org/0000-0001-8417-3774
Thiago Izaquiel de Farias Universidade Federal do Agreste de Pernambuco, Garanhuns, PE https://orcid.org/0000-0003-4839-0012
Iuri Andrade de Melo Universidade Federal do Agreste de Pernambuco, Garanhuns, PE https://orcid.org/0000-0001-6891-7737
Kleber Régis Santoro Universidade Federal do Agreste de Pernambuco, Garanhuns, PE https://orcid.org/0000-0002-7592-8423
Sylvia Anton UMR IGEPP INRAE/Institut Agro/Université Rennes 1, Institut Agro, Rennes, Angers https://orcid.org/0000-0001-6268-8056
César Auguste Badji Universidade Federal do Agreste de Pernambuco, Garanhuns, PE https://orcid.org/0000-0001-8082-3784

DOI:

https://doi.org/10.1590/1983-21252023v36n106rc

Keywords:

Insecticide. Control failure. Resistance monitoring.

Abstract

Plutella xylostella cause severe damage on cruciferous plants all over the world. Farmers in several regions of Brazil report increasing inefficiency of chemical control, even when using high insecticide concentrations. We therefore assume that regional populations of these insects develop multiple resistances. We evaluate here the susceptibility of brazilian diamondback moth populations to the Premio^® (Chlorantraniliprole), Dipel^® (Bacillus thuringiensis var. Kurstaki) and Lannate^® BR (Oxime Methylcarbamate) insecticides, frequently used in Brazil. Susceptibility bioassays with five field-collected and two laboratory diamondback moth populations were conducted with increasing concentrations of insecticides up to ten times above the recommended concentration. Extremely high and region-dependent resistances were found in field populations against Chlorantraniliprole and B. thuringiensis, with Resistance Ratios up to 370.0 times for certain populations. For Oxime Methylcarbamate, we were not able to do Probit analyses for the field populations because of very low mortality rates. Laboratory populations showed resistance to the three tested insecticides with all LC₅₀concentrations exceeding the recommended doses by at least 3 times. Our results show strong and variable resistance to the three tested insecticides according to the region of origin. To maintain efficient pest control in a large country like Brazil, local levels of resistance need therefore to be monitored by the authorities and indications for concentrations of insecticides to be used in the field should be adjusted to each region, to prevent massive spread of insecticides in the field.

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Author Biography

José Gomes da Silva Filho, Department of Phytossanity, Universidade Federal de Pelotas, Pelotas, RS

ORCID 0000-0001-8417-3774

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