Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
DOI:
https://doi.org/10.1590/1983-21252023v36n214rcKeywords:
Anthocyanins. Chlorophyll. Lamiaceae. Micropropagation.Abstract
Melissa officinalis L., popularly known as lemon balm, is an aromatic plant widely used in medicine, cosmetics, and pharmaceutical industries for its essential oil rich in phenylpropanoids, terpenes, and phenolics. This study aimed to assess the effect of growth regulators on the development and physiological and biochemical metabolism of M. officinalis cultured in vitro. Seeds were inoculated in Murashige and Skoog medium and added with the regulators 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) according to six different treatments. After 90 days of culture, plants were evaluated for growth and biochemical and physiological parameters (flavonoids, anthocyanins, and chlorophyll). The balance between regulators interfered with plant growth, which increased in the presence of 0.2 mg L−1 BAP. In this treatment, the plants had greater growth with more leaves, and the biomass production of shoots and roots was higher than the control. Growth regulators did not influence nitrogen assimilation or flavonoid production; however, total chlorophyll and anthocyanin indexes were enhanced by treatment with BAP at concentrations ranging from 0.2 to 0.5 mg L−1. Auxin treatment did not improve root production or growth but favored callus formation when combined with 0.5–3.0 mg L−1 BAP. The results indicate that high BAP concentrations (above 1.0 mg L−1) should not be used in in vitro production of lemon balm.
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ALONI, R. et al. Root-synthesized cytokinin in Arabidopsis is distributed in the shoot by the transpiration stream. Journal of Experimental Botany, 56: 1535-1544, 2005.
ALVAREZ, M. A. Plant biotechnology for health: from secondary metabolites to molecular Farming. 1. ed. Buenos Aires: Springer, 2014. 161 p.
ASGHARI, F. et al. Effect of explants source and different hormonal combinations on direct regeneration of basil plants (Ocimum basilicum L.). Australian Journal of Agricultural Engineering, 3: 12-17, 2012.
ASMAR, A. S. et al. Citocininas na multiplicação in vitro de hortelã-pimenta (Mentha piperita L.). Revista Brasileira de Plantas Medicinais, 13: 533-538, 2011.
BARBOSA, M. R. et al. Plant generation and enzymatic detoxification of reactive oxygen species. Ciência Rural, 44: 453-460, 2014.
CARVALHO, F.; DUARTE, A. P.; FERREIRA, S. Antimicrobial activity of Melissa officinalis and its potential use in food preservation. Food Bioscience, 44: 1-14, 2021.
CHATTERJEE, T.; GHOSH, B. Micropropagation of medicinal plants: A review. International Journal of Economic Plants, 7: 66-72, 2020.
FERRARI, M. P. S. et al. Growth regulators affect the growth and biochemical activity of Curcuma longa plants grown in vitro. Journal of Agricultural Science, 11: 277-291, 2019.
FERREIRA, D. F. A computer statistical analysis system. Ciência e Agrotecnologia, 359: 1039-1042, 2011.
GILL, S. S.; TUTEJA, N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909-930, 2010.
GÓRSKI, F.; GEROTTI, G. M.; MAGALHÃES, H. M. Relationship between auxins and cytokinins in the growth and organogenesis of Ocimum basilicum L.‘Grecco a Palla’. Canadian Journal of Plant Science, 101: 698-713, 2021.
GREENWAY, M. B. et al. A nutrient medium for diverse applications and tissue growth of plant species in vitro. In Vitro Cellular & Developmental Biology-Plant, 48: 403-410, 2012.
JAN, T. et al. Range of factors in the reduction of hyperhydricity associated with in vitro shoots of Salvia santolinifolia Bioss. Brazilian Journal of Biology, 83: 1-8, 2021.
LATTANZIO, V. Phenolic Compounds:Introduction 50. Natural Producs, s/v:1543-1580, 2013.
LUZ, J. M. Q. et al. Produção de óleo essencial de Melissa officinalis L. em diferentes épocas, sistemas de cultivo e adubações. Revista Brasileira Plantas Medicinais, 16: 552-560, 2014.
MACHADO, M. P.; SILVA, A. L. L.; BIASI, L. A. Effect of plant growth regulators on in vitro regeneration of Lavandula dentata L. shoot tips. Journal of Biotechnology and Biodiversity, 2: 28-31, 2011.
MATKOWSKI, A. Plant in vitro culture for the production of antioxidants-a review. Biotechnology Advances, 26: 548-560, 2008.
MEFTAHIZADE, H.; LOTFI, M.; MORADKHANI, H.; Optimization of micropropagation and establishment of cell suspension culture in Melissa officinalis L. African Journal of Biotchnology, 9: 4314-4321, 2010.
MIRAJ, S.; RAFIEIAN, K.; KIANI, S. Melissa officinalis L: A Review study with an antioxidant prospective. Journal of Evidence-Based Complementary & Alternative Medicine, 22: 385-394, 2017.
MONFORT, L. E. F. et al. Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum. Industrial Crops and Products, 116: 231-239, 2018.
MORADKHANI, H. et al. Melissa officinalis L., a valuable medicine plant: a review. Journal of Medicinal Plants Research, 4: 2753-2759, 2010.
MORAIS, T. P.; ASMAR, S. A.; LUZ, J. M. Q. Reguladores de crescimento vegetal no cultivo in vitro de mentha x piperita. Revista Brasileira de Plantas Medicinais, 16: 350-355, 2014.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497, 1962.
NEELAKANDAN, A. K.; WANG, K. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant Cell Reports, 31: 597-620, 2012.
PHILLIPS, G. C.; GARDA, M. Plant tissue culture media and practices: an overview. In Vitro Cellular & Developmental Biology-Plant, 55: 242-257, 2019.
PRUDENTE, D. O.; SOUZA, L. B.; PAIVA, R. Plant somatic embryogenesis: Modulatory role of oxidative stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 90: 483-487, 2020.
RANI, K. Role of antioxidants in prevention of diseases. Journal of Applied Biotechnology Bioengineering, 4: 1-2, 2017.
REIS, E. S. et al. Influência do meio de cultura na germinação de sementes in vitro e taxa de multiplicação de Melissa officinalis L. Revista Ceres, 55: 160-167, 2008.
SANTORO, V. M. et al. Effects of growth regulators on biomass and the production of secondary metabolites in peppermint (Mentha piperita) micropropagated in vitro. American Journal of Plant Sciences, 4:49-55, 2013.
SHAKERI, A.; SAHEBKAR, A.; JAVADI, B. Melissa officinalis L. a review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology, 188: 204-228, 2016.
SMALL, C. C. et al. Plant growth regulators for enhancing revegetation success in reclamation: A review. Ecological Engineering, 118: 43-51, 2018.
STATSOFT, 2017. Statistica for Windows (computer program manual). Disponível em: . Acesso em: 01 set. 2021.
STIRBET, A. et al. Chlorophyll a fluorescence induction: can just a one-second measurement be used to quantify abiotic stress responses? Photosynthetica, 56: 86-104, 2018.
SZECHYŃSKA-HEBDA, M. et al. The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiologiae Plantarum, 29: 327-337, 2007.
TRETTEL, J. R. et al. In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L.‘Alfavaca Green’stem apexes. Agronomy Research, 18: 603-618, 2020.
TRETTEL, J. R. et al. 'In vitro' organogenesis and growth of 'Ocimum basilicum' 'Genovese' (basil) cultivated with growth regulators. Australian Journal of Crop Science, 13: 1131-1140, 2019.
VASCONCELOS, A. G. V. D. et al. Hyperhydricity: a metabolic disorder. Ciência Rural, 42: 837-844, 2012.
WELZ, V. F. F. et al. Growth, enzymatic activity, and antioxidant activity of sweet basil grown in vitro. Revista Caatinga, 33: 660-670, 2020.
ZUZARTE, M. R. et al. Trichomes, essential oils and in vitro propagation of Lavandula pedunculata (Lamiaceae). Industrial Crops and Products, 32: 580-587, 2010.
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