In vitro effect of polar extracts from Annona glabra seeds on Fusarium solani

Oscar Marino Mosquera Martínez; Ana Isabel Giraldo Rivera; Gloria Edith Guerrero Álvarez; Norma Patricia Durán Osorio; Nathalia Cardona Hurtado

doi:10.1590/1983-21252024v3711810rc

Authors

Oscar Marino Mosquera Martínez Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0003-2998-0842
Ana Isabel Giraldo Rivera Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-4733-5460
Gloria Edith Guerrero Álvarez Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-0529-5835
Norma Patricia Durán Osorio Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-2711-6842
Nathalia Cardona Hurtado Universidad Tecnológica de Pereira, Pereira, Risaralda, Colombia https://orcid.org/0000-0002-3814-4726

DOI:

https://doi.org/10.1590/1983-21252024v3711810rc

Keywords:

Annonaceae. Acetogenins. Alkaloids. Biological control. Terpenoids.

Abstract

Fusarium species are the cause of diseases in various crops throughout the world and cause significant economic losses. Chemical fungicides are the main strategy for control, but their intensive and long-term use generates resistance, toxic residues, and environmental contamination. The biological control of bioactive substances has wide applications. In this study, the fungicidal activity of the extract obtained from Annona glabra L. seeds was evaluated as a control alternative for Fusarium solani. In addition, chemical characterization was carried out to identify the groups of metabolites present in the extract. The fungicidal activity against F. solani was evaluated using the microdilution method, and the minimum inhibitory concentration (MIC) was established, as well as the mean inhibitory concentration (IC₅₀). The IC₅₀ of the extract was 972.24 mg L⁻¹, and the MIC was 12.02 mg L⁻¹. Using chemical characterization by ultrahigh-efficiency liquid chromatography coupled with mass spectrometry, the presence of THF acetogeniones, isoquinolinic alkaloids, and kaurene-type diterpenes, which were the main compounds responsible for the activity evaluated, was confirmed. The results obtained provide evidence that A. glabra seed extracts can be a potential source of metabolites with fungicidal activity.

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