EXOPOLYSACCHARIDES AND ABIOTIC STRESS TOLERANCE IN BACTERIAL ISOLATES FROM “SABIÁ” NODULES

Authors

  • Cybelle Souza Oliveira Universidade Federal Rural de Pernambuco
  • Mario Andrade Lira Junior Universidade Federal Rural de Pernambuco
  • Newton Pereira Stamford Universidade Federal Rural de Pernambuco
  • Júlia Kuklinsky-Sobral Universidade Federal Rural de Pernambuco
  • Fátima Maria Souza Moreira Universidade Federal de Lavras

Keywords:

Acidity. Aluminum. Salinity. Antibiotics. Herbicides.

Abstract

Several microorganisms produce polysaccharides, deemed to protect the bacteria from several environmental stresses. This paper aims to evaluate the protective effect of exopolyssacharides to different abiotic stresses in bacterial isolates from “sabiá” (Mimosa caesalpiniifolia) nodules. 303 fast growing isolates were qualitatively evaluated for exopolyssacharide production and tested in vitro for tolerance to two levels of acidity, joint aluminum and acidity, three salinity levels; 11 antibiotics and three herbicides. Most isolates resisted media acidity, acidity with aluminum, salinity, and ampicillin, cefotaxime, gentamicin and vancomycin antibiotics and 2,4D herbicide, while being sensitive to ciprofloxacin, chloramphenicol, streptomycin, kanamycin, nalidixic acid, rifampicin and tetracycline antibiotics and paraquat and glyphosate herbicides. There was no connection between exopolyssacharide production and abiotic stress tolerance.

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References

AHEMAD, M.; KHAN, M. S. Effects of pesticides on plant growth promoting traits of Mesorhizobium strain MRC4. Journal of the Saudi Society of Agricultural Sciences, v. 11, n. 1, p. 63-71, 2012.

BAUER, A. W.; KIRBY, W. M. M.; SHERRIS, J. C. Antibiotic susceptibility testing by standardized single disk method. American Journal of Clinical Pathology, v. 45, n. 4, 1966.

BOMFETI, C. A. et al. Exopolysaccharides produced by the symbiotic nitrogen-fixing bacteria of leguminosae. Revista Brasileira de Ciência do Solo, Viçosa, v. 35, n. 3, p. 657-671, 2011.

CHAGAS JÚNIOR, A. F.; OLIVEIRA, L. A.; OLIVEIRA, A. N. Caracterização fenotípica de rizóbio nativos isolados de solos da Amazônia e eficiência simbiótica em feijão caupi. Acta Scientiarum.Agronomy, Cidade, v. 32, n. 1, p. 161-169, 2010.

CORREA, O. S.; BARNEIX, A. J. Cellular mechanisms of pH tolerance in Rhizobium loti. World Journal of Microbiology and Biotechnology, v. 13, n. 2, p. 153-157, 1997.

CUNNINGHAM, S. D.; MUNNS, D. N. The correlation between extracellular polysaccharide production and acid tolerance in Rhizobium. Soil Science Society of America Journal, v. 48, n. 6, p. 1273-1276, 1984.

DOWNIE, J. A. The roles of extracellular proteins, polysaccharides and signals in the interactions of rhizobia with legume roots. FEMS Microbiology Reviews, v. 34, n. 2, p. 150-170, 2010.

ELSHEIKH, E. A. E. Effects of salt on rhizobia and bradyrhizobia: A review. Annals of Applied Biology, v. 132, p. 507-524, 1998.

FARELEIRA, P.; MATOS, N.; FERREIRA, E.; MARQUES, J. F. Tolerância ao sal e às altas temperaturas de estirpes de Sinorhizobium provenientes de zonas secas do Alentejo. Revista de Ciências Agrárias, Belém, v. 30, n. 2, p. 187-198, 2007.

FLORENTINO, L. A. et al. Diversity and efficiency of Bradyrhizobium strains isolated from soil samples collected from around Sesbania virgata roots using cowpea as trap species. Revista Brasileira de Ciência do Solo, Viçosa, v. 34, n. 4, p. 1113-1123, 2010.

FRED, E. B.; WAKSMAN, S. A. Laboratory manual of general microbiology - with special reference to the microorganisms of the soil. New York: McGraw-Hill, 1928. 143 p.

GAURI, A. K. S. et al. Characterization of Rhizobium isolated from root nodules of Trifolium alexandrinum. Journal of Agricultural Technology, v. 7, n. 6, p. 1705-1723, 2011.

HOSNEY, I. et al. Intrinsic Antibiotic Resistance (IAR) for differentiation of rhizobia. Scientific Research Outlook & Technology Development in the Arab World 4, 2006, Damascus. Arab Science & Technology Foundation. p. 11-14.

JACQUES, R. J. S. et al. Sensibilidade de estirpes de Bradyrhizobium ao glyphosate. Revista Ceres, Viçosa, v. 57, n. 1, p. 28-33, 2010.

JANCZAREK, M.; SKORUPSKA, A. Modulation of rosR Expression and Exopolysaccharide Production in Rhizobium leguminosarum bv. trifolii by Phosphate and Clover Root Exudates. International Journal of Molecular Sciences, v. 12, n. 6, p. 4132-4155, 2011.

MAÂTALLAH, J. et al. Phenotypic and molecular characterization of chickpea rhizobia isolated from different areas of Morocco. Journal of Applied Microbiology, v. 93, n. 4, p. 531-540, 2002.

MALTY, J. S.; SIQUEIRA, J. O.; MOREIRA, F. M. S. Efeitos do glifosato sobre microrganismos simbiotróficos de soja, em meio de cultura e casa de vegetação. Pesquisa Agropecuária Brasileira, Brasília, v. 41, n. 2, p. 285-291, 2006.

NICOLAUS, B.; KAMBOUROVA, M.; ONER, E. T. Exopolyssacharides from extremophiles: from fundamentals to biotechnology. Environmental Technology, v. 31, n. 1, p. 1145-1158, 2010.

NÓBREGA, R. S. A. et al. Tolerância de bactérias diazotróficas simbióticas à salinidade in vitro. Ciência e Agrotecnologia, Lavras, v. 28, n. 4, p. 899-905, 2004.

REJILI, M. et al. Genetic diversity of rhizobial populations recovered from three Lotus species cultivated in the infra-arid Tunisian soils. Progress in Natural Science, v. 19, n. 9, p. 1079-1087, 2009.

SÁ, E. L. S. Diversidade fenotípica e genética de rizóbios isolados de feijoeiro (phaseolus vulgaris) em solos ácidos de Cunha - SP. 2001. 109 f. Tese (Doutorado em Ciência - Energia Nuclear na Agricultura) - Centro de Energia Nuclear na Agricultura, USP, Piracicaba, 2001.

SANTOS, J. B. et al. Efeitos de diferentes formulações comerciais de glyphosate sobre estirpes de Bradyrhizobium. Planta Daninha, Viçosa, v. 22, n. 2, p. 293-299, 2004.

SANTOS, J. B. et al. Ação de herbicidas sobre o crescimento de estirpes de Rhizobium tropici. Planta Daninha, Viçosa, v. 24, n. 3, p. 457-465, 2006.

SHENDE, R. C.; PATIL, M. B. Effects of glyphosate on metabolism of phenolic compounds (PAL). Journal of Medical Pharmaceutical and Allied Sciences, v. 2013, n. 4, p. 25-51, 2013.

STAUDT, A.; WOLFE, L.; SHROUT, J. Variations in exopolysaccharide production by Rhizobium tropici. Archives of Microbiology, v. 194, n. 3, p. 197-206, 2012.

SURESH KUMAR, A.; MODY, K.; JHA, B. Bacterial exopolysaccharides – a perception. Journal of Basic Microbiology, v. 47, n. 2, p. 103-117, 2007.

VEENA, K. et al. Influence of herbicides on Rhizobium growth and its symbiosis with pigeonpea. Trends in Biosciences, v. 5, n. 2, p. 133-135, 2012.

VINCENT, J. M. A manual for the practical study of root-nodule bacteria. Oxford: Blackwell, 1970.

WILDER, M. A. et al. Normas de Desempenho para Testes de Sensibilidade Antimicrobiana; 15o Suplemento Informativo. M100-S15. Brasília: Agência Nacional de Vigilância Sanitária, 2005. (CLINICAL AND LABORATORY STANDARDS INSTITUTE)

XAVIER, G. R. et al. Edaphic factors as determinants for the distribution of intrinsic antibiotic resistance in a cowpea rhizobia population. Biology and Fertility of Soils, v. 27, n. 4, p. 386-392, 1998.

XAVIER, G. R. et al. Tolerância de rizóbio de feijão-caupi à salinidade e à temperatura em condição in vitro. Revista Caatinga, Mossoró, v. 20, n. 4, p. 1-9, 2007.

Published

02-12-2014

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Section

Technical Note