RELATIVE EXPRESSION OF GENES CHIA1, SGF14C AND CHS8* IN SOYBEAN SEED COATS
Keywords:
Glycine max. Protection structure. Candidate genes. Seed quality.Abstract
The aim of this study was to evaluate the relative expression of three candidate genes, CHIA1, SGF14c and CHS8 * possibly involved in seed quality, in contrasting seed coats from four soybean genotypes. Two genotypes with yellow seed coats, BMX Potência RR and CD 202, and two genotypes with black seed coats, TP and IAC were studied to determine the relative gene expression through the qPCR technique, in seven stages of seed coat development for all four genotypes, at 25, 30, 35, 40, 45, 50 and 55 days after anthesis. The CHIA1 and SGF14c genes showed higher expression in cultivar CD 202; the former in the final stages of seed coat development, at 55 days after anthesis, the latter gene at earlier stages, specifically at 25 days after anthesis. The CHS8* gene showed higher expression in CD 202 seed coats at 50 days after anthesis. All three genes expressed at higher levels on genotypes of yellow seed coats, and are considered relevant to new areas of research based on the expression of genes related to seed quality.Downloads
Download data is not yet available.
References
CAPELETI, I. et al. A new procedure for the quantification of lignin in soybean (Glycine max (L.) Merrill) seed coat and their relationship with the resistance to mechanical damage. Seed Science and Technology, Zurich, v. 33, n. 2, p. 511–515, 2005.
CHO, Y. B.; JONES, S. I.; VODKIN, L. The transition from primary sirnas to amplified secondary sirnas that regulate chalcone synthase during development of Glycine max seed coats. PloS One, Califórnia, v. 8, n. 10, p. 1-10, 2013.
DAO, T. T. H.; LINTHORST, H. J. M.; VERPOORTE, R. Chalcone synthase and its functions in plant resistance. Phytochemistry Reviews, Dordrecht, v. 10, n. 3, p. 397–412, 2011.
DHAUBHADEL, S.; LI, X. A new client for 14-3-3 proteins: GmMYB176, an R1 MYB transcription factor. Plant Signaling & Behavior, Texas, v. 5, n. 3, p. 921-923, 2010.
DHAUBHADEL, S. et al. Transcriptome analysis reveals a critical role of CHS7 and CHS8 genes for isoflavonoid synthesis in soybean seeds. Plant Physiology, Washington, v. 143, n. 1, p. 326–338, 2007.
DURRANT, W. E.; DONG, X. Systemic acquired resistance. Annual Review of Phytopathology, Califórnia, v. 42, p. 185-209, 2004.
GIJZEN, M. et al. A class I chitinase from soybean seed coat. Journal of Experimental Botany, Oxford, v. 52, n. 365, p. 2283–2289, 2001.
HAJDUCH, M. et al. A systematic proteomic study of seed filling in soybean. Establishment of high-resolution two-dimensional reference maps, expression profiles, and an interactive proteome database. Plant Physiology, Washington, v. 137, n. 4, p. 1397–1419, 2005.
KASPRZEWSKA, A. Plant chitinases–regulation and function. Cellular & Molecular Biology Letters, Wroclaw, v. 8, p. 809–824, 2003.
KERN, M. F. et al. Expression of a chitinase gene from Metarhizium anisopliae in tobacco plants confers resistance against Rhizoctonia solani. Applied Biochemistry and Biotechnology, Clifton, v. 160, n. 7, p. 1933–1946, 2010.
KURAUCHI, T. et al. Endogenous short interfering RNAs of chalcone synthase genes associated with inhibition of seed coat pigmentation in soybean. Breeding Science, Tokyo, v. 59, n. 4, p. 419–426, 2009.
LI, X.; DHAUBHADEL, S. Soybean 14-3-3 gene family: identification and molecular characterization. Planta, Berlin, v. 233, n. 3, p. 569-582, 2011.
LI, C. et al. Arabidopsis TCP20 links regulation of growth and cell division control pathways. Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 102, n. 36, p. 12.978-12.983, 2005.
MACHADO, A. A.; CONCEIÇÃO, A. R. Sistema de análise estatística para Windows. Winstat – versão 2.0. Pelotas: UFPel, 2003.
MERTZ, L. M. et al. cDNA-AFLP analyses between black and yellow soybean seed coats. Seed Science and Technology, Zurich, v. 38, n. 1, p. 88-95, 2010.
MERTZ, L. M. et al. Diferenças estruturais entre tegumentos de sementes de soja com permeabilidade contrastante. Revista Brasileira de Sementes, Brasília, v. 31, n. 1, p. 23-29, 2009.
MOÏSE, J. A. et al. Seed coats: structure, development, composition, and biotechnology. In Vitro Cellular & Developmental Biology – Plant, Columbia, v. 41, n. 5, p. 620-644, 2005.
PAUL, A. L.; FOLTA, K. M.; FERL, R. J. 14-3-3 proteins, red light and photoperiodic flowering: A point of connection? Plant Signal & Behavior, Texas, v. 3, n. 8, p. 511–515, 2008.
PFAFFL, M. W. A new mathematical model for relative quantification in real time RT – PRC. Nucleic Acids Research, Oxford, v. 29, n. 9, p. 2003-2007, 2001.
PIGNOCCHI, C.; DOONAN, J. H. Interaction of a 14-3-3 protein with the plant microtubule - associated protein EDE1. Annals of Botany, London, v. 107, n. Special, p. 1103-1109, 2011.
PULLA, R. K. et al. Identification and characterization of class I chitinase in Panax ginseng C.A. Meyer. Molecular Biology Reports, Dordrecht, v. 38, n. 1, p. 95–102, 2011.
RADWAN, O. et al. 14-3-3 proteins SGF14c and SGF14l play critical roles during soybean nodulation. Plant Physiology, Washington, v. 160, n. 4, p. 2125-2136, 2012.
SCHOONHEIM, P. J.; PEREIRA, D. da C.; BOER, A. H. de. Dual role for14-3-3 proteins and ABF transcription factors in gibberellic acid and abscisic acid signalling in barley (Hordeum vulgare) aleurone cells. Plant, Cell & Environmental, Massachusetts, v. 32, n. 5, p. 439-447, 2009.
SENDA, M. et al. Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes. The Plant Cell, Washington, v. 16, n. 4, p. 807–818, 2004.
TUTEJA, J. H.; VODKIN, L. O. Structural features of the endogenous CHS silencing and target loci in the soybean genome. Crop Science, Madison, v. 48, n. 1, p. S-49-S-68, 2008.
TUTEJA, J. H. et al. O. Tissue-specific gene silencing mediated by a naturally occurring chalcone synthase gene cluster in Glycine max. The Plant Cell, Washington, v. 16, n. 4, p. 819–835, 2004.
VOINNET, O. Induction and suppression of RNA silencing: insights from viral infections. Nature Reviews Genetics, London, v. 6, p. 206-220, 2005.
XU, W. F.; SHI, W. M. Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots: analysis by real-time RT-PCR. Annals of Botany, London, v. 98, n. 5, p. 965–974, 2006.
WEBB, D. M. et al. Genetic mapping of soybean cyst nematode race-3 resistance loci in the soybean PI 437654. Theoretical & Applied Genetics, Berlin, v. 91, n. 4, p. 574–581, 1995.
YANG, X. et al. Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by supression substractive hybridization and macroarray. Journal of Experimental Botany, Lancaster, v. 59, n. 13, p. 3661-3674, 2008.
ZHAO, S.; FERNALD, R. D. Comprehensive algorithm for quantitative real-time polymerase chain reaction. Journal of Computational Biology, New York, v. 12, n. 8, p. 1047-1064, 2005.
CHO, Y. B.; JONES, S. I.; VODKIN, L. The transition from primary sirnas to amplified secondary sirnas that regulate chalcone synthase during development of Glycine max seed coats. PloS One, Califórnia, v. 8, n. 10, p. 1-10, 2013.
DAO, T. T. H.; LINTHORST, H. J. M.; VERPOORTE, R. Chalcone synthase and its functions in plant resistance. Phytochemistry Reviews, Dordrecht, v. 10, n. 3, p. 397–412, 2011.
DHAUBHADEL, S.; LI, X. A new client for 14-3-3 proteins: GmMYB176, an R1 MYB transcription factor. Plant Signaling & Behavior, Texas, v. 5, n. 3, p. 921-923, 2010.
DHAUBHADEL, S. et al. Transcriptome analysis reveals a critical role of CHS7 and CHS8 genes for isoflavonoid synthesis in soybean seeds. Plant Physiology, Washington, v. 143, n. 1, p. 326–338, 2007.
DURRANT, W. E.; DONG, X. Systemic acquired resistance. Annual Review of Phytopathology, Califórnia, v. 42, p. 185-209, 2004.
GIJZEN, M. et al. A class I chitinase from soybean seed coat. Journal of Experimental Botany, Oxford, v. 52, n. 365, p. 2283–2289, 2001.
HAJDUCH, M. et al. A systematic proteomic study of seed filling in soybean. Establishment of high-resolution two-dimensional reference maps, expression profiles, and an interactive proteome database. Plant Physiology, Washington, v. 137, n. 4, p. 1397–1419, 2005.
KASPRZEWSKA, A. Plant chitinases–regulation and function. Cellular & Molecular Biology Letters, Wroclaw, v. 8, p. 809–824, 2003.
KERN, M. F. et al. Expression of a chitinase gene from Metarhizium anisopliae in tobacco plants confers resistance against Rhizoctonia solani. Applied Biochemistry and Biotechnology, Clifton, v. 160, n. 7, p. 1933–1946, 2010.
KURAUCHI, T. et al. Endogenous short interfering RNAs of chalcone synthase genes associated with inhibition of seed coat pigmentation in soybean. Breeding Science, Tokyo, v. 59, n. 4, p. 419–426, 2009.
LI, X.; DHAUBHADEL, S. Soybean 14-3-3 gene family: identification and molecular characterization. Planta, Berlin, v. 233, n. 3, p. 569-582, 2011.
LI, C. et al. Arabidopsis TCP20 links regulation of growth and cell division control pathways. Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 102, n. 36, p. 12.978-12.983, 2005.
MACHADO, A. A.; CONCEIÇÃO, A. R. Sistema de análise estatística para Windows. Winstat – versão 2.0. Pelotas: UFPel, 2003.
MERTZ, L. M. et al. cDNA-AFLP analyses between black and yellow soybean seed coats. Seed Science and Technology, Zurich, v. 38, n. 1, p. 88-95, 2010.
MERTZ, L. M. et al. Diferenças estruturais entre tegumentos de sementes de soja com permeabilidade contrastante. Revista Brasileira de Sementes, Brasília, v. 31, n. 1, p. 23-29, 2009.
MOÏSE, J. A. et al. Seed coats: structure, development, composition, and biotechnology. In Vitro Cellular & Developmental Biology – Plant, Columbia, v. 41, n. 5, p. 620-644, 2005.
PAUL, A. L.; FOLTA, K. M.; FERL, R. J. 14-3-3 proteins, red light and photoperiodic flowering: A point of connection? Plant Signal & Behavior, Texas, v. 3, n. 8, p. 511–515, 2008.
PFAFFL, M. W. A new mathematical model for relative quantification in real time RT – PRC. Nucleic Acids Research, Oxford, v. 29, n. 9, p. 2003-2007, 2001.
PIGNOCCHI, C.; DOONAN, J. H. Interaction of a 14-3-3 protein with the plant microtubule - associated protein EDE1. Annals of Botany, London, v. 107, n. Special, p. 1103-1109, 2011.
PULLA, R. K. et al. Identification and characterization of class I chitinase in Panax ginseng C.A. Meyer. Molecular Biology Reports, Dordrecht, v. 38, n. 1, p. 95–102, 2011.
RADWAN, O. et al. 14-3-3 proteins SGF14c and SGF14l play critical roles during soybean nodulation. Plant Physiology, Washington, v. 160, n. 4, p. 2125-2136, 2012.
SCHOONHEIM, P. J.; PEREIRA, D. da C.; BOER, A. H. de. Dual role for14-3-3 proteins and ABF transcription factors in gibberellic acid and abscisic acid signalling in barley (Hordeum vulgare) aleurone cells. Plant, Cell & Environmental, Massachusetts, v. 32, n. 5, p. 439-447, 2009.
SENDA, M. et al. Patterning of virus-infected Glycine max seed coat is associated with suppression of endogenous silencing of chalcone synthase genes. The Plant Cell, Washington, v. 16, n. 4, p. 807–818, 2004.
TUTEJA, J. H.; VODKIN, L. O. Structural features of the endogenous CHS silencing and target loci in the soybean genome. Crop Science, Madison, v. 48, n. 1, p. S-49-S-68, 2008.
TUTEJA, J. H. et al. O. Tissue-specific gene silencing mediated by a naturally occurring chalcone synthase gene cluster in Glycine max. The Plant Cell, Washington, v. 16, n. 4, p. 819–835, 2004.
VOINNET, O. Induction and suppression of RNA silencing: insights from viral infections. Nature Reviews Genetics, London, v. 6, p. 206-220, 2005.
XU, W. F.; SHI, W. M. Expression profiling of the 14-3-3 gene family in response to salt stress and potassium and iron deficiencies in young tomato (Solanum lycopersicum) roots: analysis by real-time RT-PCR. Annals of Botany, London, v. 98, n. 5, p. 965–974, 2006.
WEBB, D. M. et al. Genetic mapping of soybean cyst nematode race-3 resistance loci in the soybean PI 437654. Theoretical & Applied Genetics, Berlin, v. 91, n. 4, p. 574–581, 1995.
YANG, X. et al. Expression profile analysis of genes involved in cell wall regeneration during protoplast culture in cotton by supression substractive hybridization and macroarray. Journal of Experimental Botany, Lancaster, v. 59, n. 13, p. 3661-3674, 2008.
ZHAO, S.; FERNALD, R. D. Comprehensive algorithm for quantitative real-time polymerase chain reaction. Journal of Computational Biology, New York, v. 12, n. 8, p. 1047-1064, 2005.
Downloads
Published
02-12-2014
Issue
Section
Agronomy
License
Os Autores que publicam na Revista Caatinga concordam com os seguintes termos:
a) Os Autores mantêm os direitos autorais e concedem à revista o direito de primeira publicação, com o trabalho simultaneamente licenciado sob a Licença Creative Commons do tipo atribuição CC-BY, para todo o conteúdo do periódico, exceto onde estiver identificado, que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista, sem fins comerciais.
b) Os Autores têm autorização para distribuição não-exclusiva da versão do trabalho publicada nesta revista (ex.: publicar em repositório institucional ou como capítulo de livro), com reconhecimento de autoria e publicação inicial nesta revista.
c) Os Autores têm permissão e são estimulados a publicar e distribuir seu trabalho online (ex.: em repositórios institucionais ou na sua página pessoal) a qualquer ponto antes ou durante o processo editorial, já que isso pode gerar alterações produtivas, bem como aumentar o impacto e a citação do trabalho publicado (Veja O Efeito do Acesso Livre).
