RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO

Authors

  • Ronan Carlos Colombo Center of Agrarian Science, Department of Agronomy, Universidade Estadual de Londrina, Londrina, PR
  • Vanessa Favetta Center of Agrarian Science, Department of Agronomy, Universidade Estadual de Londrina, Londrina, PR
  • Ricardo Tadeu de Faria Center of Agrarian Science, Department of Agronomy, Universidade Estadual de Londrina, Londrina, PR
  • Felipe Aranha de Andrade Center of Agrarian Science, Department of Agronomy, Universidade Estadual de Londrina, Londrina, PR
  • Vanderli Marino Melem Center of Exact Sciences, Department of Statistic, Universidade Estadual de Londrina, Londrina-PR

DOI:

https://doi.org/10.1590/1983-21252016v29n103rc

Keywords:

Micropropagation, Culture medium, Amorphous silica.

Abstract

Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.

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Published

10-03-2016

Issue

Section

Agronomy