IDENTIFICATION AND EFFECTS OF MIXED INFECTION OF Potyvirus ISOLATES WITH Cucumber mosaic virus IN CUCURBITS
DOI:
https://doi.org/10.1590/1983-21252016v29n429rcKeywords:
Papaya ringspot virus. Zucchini yellow mosaic virus. Watermelon mosaic virus. Synergistic interaction.Abstract
Mixed infections in cucurbits are frequently observed in natural conditions between viruses from the Potyvirus genus and Cucumber mosaic virus (CMV), which significantly decreases productivity. The objectives of the present study was to compare the host range of PRSV-W, WMV, and ZYMV isolates and evaluate the effects of mixed infections with CMV in zucchini plants (Cucurbita pepo L.). Host range studies comprising 23 plant species confirmed some similarities and biological differences among the isolates of PRSV-W, ZYMV, and WMV. RT-PCR confirmed the amplification of DNA fragments of the PRSV-W, WMV, and ZYMV coat protein gene (cp) and cytoplasm inclusion gene (ci). The virus interaction studies in zucchini Caserta plants indicated synergistic interactions, particularly among species from the Potyvirus genus, and some CMV interference with some virus combinations.Downloads
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References
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MOWAT, W. P.; DAWSON, S. Detection of plant viruses by ELISA using crude sap extracts and unfractionated antisera. Journal of Virological Methods, Amsterdam, v. 15, n. 3, p. 233-247, 1987.
MURPHY, J. F.; BOWEN, K. L. Synergistic disease in pepper caused by the mixed infection of Cucumber mosaic virus and Pepper mottle virus. Phytopathology, Saint Paul, v. 96, n. 3, p. 240-247, 2006.
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SILVEIRA, L. M. et al. Levantamento sorológico de vírus em espécies de cucurbitáceas na região do submédio São Francisco, Brasil. Tropical Plant Pathology, Brasília, v. 34, n. 2, p. 123-126, 2009.
YAKOUBI, S. et al. Molecular, biological and serological variability of Zucchini yellow mosaic virus in Tunisia. Plant Pathology, Oxford, v. 57, n. 6, p. 1146-1154, 2008.
BARBA, M.; JELKMAN, W.; MARTIN, R. Detection of virus and virus-like diseases of fruit trees and small fruit crops. Acta Horticulturae, The Hague, v. 2, n. 472, p. 759-783, 1998.
CHOI, S. K. et al. Systemic movement of a movement-deficient strain of Cucumber mosaic virus in zucchini squash is facilitated by a cucurbit-infecting potyvirus. Journal of General Virology, London, v. 83, n. 12, p. 3173-3178, 2002.
CHOMCZYNSKI, P.; SACCHI, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, San Diego, v. 162, n. 1, p. 156-159, 1987.
COUTTS, B. A. et al. Zucchini yellow mosaic virus: biological properties, detection procedures and comparison of coat protein gene sequences. Archives of Virology, Wien, v. 156, n. 12, p. 2119-2131, 2011.
CRUZ, C. D. GENES - a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum, Maringá, v. 35, n. 3, p. 271-276, 2013.
DESBIEZ, C.; LECOQ, H. Zucchini yellow mosaic virus. Plant Pathology, Oxford, v. 46, n. 6, p. 809-829, 1997.
FINETTI-SIALER, M. M. et al. Biological and molecular characterization of a recombinant isolate of Watermelon mosaic virus associated with a watermelon necrotic disease in Italy. European Journal of Plant Pathology, Dordrecht, v. 132, n. 3, p. 317-322, 2012.
GISI, U. Synergistic interaction of fungicides in mixtures. Phytopathology, Saint Paul, v. 86, n. 11, p. 1273-1279, 1996.
HOSSEINI, S. et al. Characterization of the Zucchini yellow mosaic virus from squash in Tehran province. Journal of Agricultural Science and Technology, Tehran, v. 9, n. 2, p. 137-143, 2007.
JUAREZ, M. et al. Relative incidence, spatial distribution and genetic diversity of cucurbit viruses in eastern Spain. Annals of Applied Biology, London, v. 162, n. 3, p. 362-370, 2013.
KIM, M. K. et al. Characteristics of Cucumber mosaic virus infecting zucchini in Korea. Plant Pathology Journal, Seoul, v. 26, n. 2, p. 139-148, 2010.
KUMAR, S.; SANKARLINGAM, A.; RABINDRAN, R. Characterization and Confirmation of Papaya ringspot virus-W strain Infecting Trichosanthese cucumerina at Tamil Nadu, India. Journal of Plant Pathology & Microbiology, Los Angeles, v. 5, n. 2, p. 225, 2014.
LIMA, J. A. A. et al. Serology Applied to Plant Virology. In: Moslih Al-Moslih. (Org.). Serological diagnosis of certain human, animal and plant diseases. Rijeka, Croatia: InTech, 1. p.71-94, 2012b.
LIMA, J. A. A. et al. Virologia essencial & viroses em culturas tropicais. 1. ed. Fortaleza, CE: Edições UFC. 2015. 605 p.
LIMA, J. A. A. et al. Viruses infecting melon and watermelon in Northeastern Brazil. Virus Reviews and Research, Rio de Janeiro, v. 17, n. 1, p. 29-35, 2012a.
MALIK, A. H. et al. Severe disease of melon in North West frontier province is associated with simultaneous infection of two RNA viruses. Pakistan Journal of Botany, Karachi, v. 42, n. 1, p. 361-367, 2010.
MOREIRA, A. S.; FILHO, A. B.; REZENDE, J. A. M. Comparative epidemiology of three virus diseases on zucchini squash. Journal of Phytopathology, Berlin, v. 163, n. 5, p. 386-394. 2015.
MOWAT, W. P.; DAWSON, S. Detection of plant viruses by ELISA using crude sap extracts and unfractionated antisera. Journal of Virological Methods, Amsterdam, v. 15, n. 3, p. 233-247, 1987.
MURPHY, J. F.; BOWEN, K. L. Synergistic disease in pepper caused by the mixed infection of Cucumber mosaic virus and Pepper mottle virus. Phytopathology, Saint Paul, v. 96, n. 3, p. 240-247, 2006.
PINTO, Z. V. et al. Ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus in single and mixed infection with two potyviruses to Zucchini Squash. Summa phytopathology, Botucatu, v. 34, n. 2, p. 183-185, 2008.
SILVA, F. R. et al. Caracterização biológica e sorológica de um isolado de Squash mosaic virus e efeitos da infecção mista com vírus do gênero Potyvirus. Revista Ciência Agronômica, Fortaleza, v. 47, n. 1, p. 195-202, 2016.
SILVEIRA, L. M. et al. Levantamento sorológico de vírus em espécies de cucurbitáceas na região do submédio São Francisco, Brasil. Tropical Plant Pathology, Brasília, v. 34, n. 2, p. 123-126, 2009.
YAKOUBI, S. et al. Molecular, biological and serological variability of Zucchini yellow mosaic virus in Tunisia. Plant Pathology, Oxford, v. 57, n. 6, p. 1146-1154, 2008.
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13-09-2016
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