MOLECULAR IDENTIFICATION OF Liriomyza sp. IN THE NORTHEAST AND SOUTHEAST REGIONS OF BRAZIL
DOI:
https://doi.org/10.1590/1983-21252017v30n409rcKeywords:
Cryptic species. DNA barcoding. Population genetics.Abstract
In Brazil, species of the genus Liriomyza are widely distributed and have economic importance as they cause damage to at least 14 plant families, especially Solanaceae, Cucurbitaceae, Asteraceae, and Fabaceae. Studies suggest existence of a species complex within this genus, based on the presence of morphological similarities among the species Liriomyza trifolii (Burgess), L. sativae Blanchard and L. huidobrensis (Blanchard). The present study aimed to use DNA barcoding to establish new distribution records of L. sativae in distinct regions in Brazil, determine intra- and inter-population genetic diversity, and reconstruct the phylogeny of Liriomyza species using the DNA barcode sequences. Identity values were between 97% and 99%, confirming that all the examined Brazilian populations belonged to the species L. sativae. Phylogenetic analyses indicated the presence of a single clade of L. sativae, composed of seven populations. Intra-population analysis on individuals of these populations indicated low levels of nucleotide and haplotype diversity. The haplotype network indicated presence of only 14 haplotypes distributed among the Brazilian populations. The genetic similarities shared by the Brazilian populations of L. sativae suggest that these populations are closely related. Genetic patterns observed among populations of L. sativae might be associated with bottleneck events or founder effect during establishment of this leafminer in Brazil.Downloads
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References
AHERN, R. G.; HAWTHORNE, D. J.; RAUPP, M. J. Founder effects and phenotypic variation in Adelges cooleyi, an insect pest introduced to the eastern United States. Biological Invasions, Boston, v. 11, n. 4, p. 951–971, 2009.
ALTSCHUL, S. F. et al. "Basic local alignment search tool". Journal of Molecular Biology, London, v. 215, n. 3, p. 403-410, 1990.
BANDELT, H. J.; ROHL, F. P. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, London, v. 16, n. 1, p. 37-48, 1999.
BLACKET, M. J. et al. DNA-based identifications reveal multiple introductions of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae) into the Torres Strait Islands and Papua New Guinea. Bulletin of Entomological Research, London, v. 105, n. 5, p. 533-544, 2015.
BUSVINE, J. R. Cryptic species of insect disease vectors and their importance. Endeavour, London, v. 4, n. 3, p. 108-112, 1980.
CLARK, A. R. et al. Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis complex of fruit Flies. Annual Review of Entomology, New York, v. 50, n. 1, p. 293-319, 2005.
COSTA-JUNIOR, C. R. L. et al. Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil. Parasites and Vectors, London, v. 8, n. 1, p. 193-202, 2015.
DE SOUZA FREITAS, M. T. et al. Phenotypic and genotypic variations among three allopatric populations of Lutzomyia umbratilis, main vector of Leishmania guyanensis. Parasites and Vectors, London, v. 8, n. 4, p. 448-458, 2015.
DELLA TORRE, A. et al. Molecular and ecological aspects of incipient speciation within Anopheles gambiae: the glass is half full. Environmental Science, Sofia, v. 289, n. 2, p. 115-117, 2002.
EDGAR, R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids resources, London, v. 32, n. 5, p. 1792-1797, 2004.
EWING, B. et al. Base-calling of automated sequencer traces using Phred. Genome research, New York, v. 8, n. 3, p. 175-185, 1998.
EXCOFFIER, L.; LISCHER H. E. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology resources, London, v. 10, n. 3, p. 564-567, 2010.
FERGUSON, J. S. Development and stability of insecticide resistance in the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to cyromazine, abamectin, and spinosad. Journal of Economic Entomology, London, v. 97, n. 1, p. 112-119, 2004.
GUINDON, S. et al. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic biology, London, v. 59, n. 3, p. 307–321, 2010.
GARIEPY, T. D. et al. Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods. Journal of applied entomology, Germany, v. 131, n. 4, p. 225–240, 2007.
HEBERT, P. D. N. et al. Biological identifications through DNA barcodes. Proceedings. Biological sciences / The Royal Society, London, v. 270, n. 1512, p. 313-321, 2003.
JENKINS, T. A.; CHAPMAN, J. L.; MICALLEF, O. L. Molecular techniques for the detection and differentiation of host and parasitoid species and the implications for fruit fly management. Journal of Insects, New York, v. 3, n. 3, p. 763–788, 2012.
KIMURA, M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, Germany, v. 16, n. 2 , p. 111–120, 1980.
KOX, L. F. F. et al. Identification of economically important Liriomyza species by PCR-RFLP analysis. Bulletin OEPP/EPPO, Paris, v. 35, n. 1, p. 79-85, 2005.
LIMA, T. C. C.; GEREMIAS, L. D.; PARRA, J. R. P. Efeito da temperatura e umidade relativa do ar no desenvolvimento de Liriomyza sativae Blanchard (Diptera: Agromyzidae) em Vigna unguiculata. Neotropical Entomology, Piracicaba, v. 38, n. 6, p. 727–733, 2009.
MURPHY, S. T.; LASALLE, J. Review article: balancing biological control strategies in the IPM of new world invasive Liriomyza leafminers in field vegetable crops. Biocontrol News and Information, New York, v. 20, n. 3, p. 91-104, 1999.
NAKANO, O.; WIENDL, F. M.; MINAMI, K. Uma nova praga (Agromyzidae) da couve. Revista Agrícola, Piracicaba, v. 42, n. 1, p. 1-10, 1967.
NEI, M. Bottlenecks, genetic polymorphism and speciation. Genetics, New York, v. 170, n. 1, p. 1-4, 2005.
NELSON, L. A.; WALLMAN J. F.; DOWTON, M. Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology, London, v. 21, n. 1, p. 44-52, 2007.
PANG, B.P. et al. Effects of different host plants on population parameters of Liriomyza sativae. Plant Protection, London, v. 31, n. 2, p. 26-28, 2005.
PARRELLA, M. P. et al. Control of Liriomyza trifolii with biological agents and insects growth regulators. California Agriculture, Richmond, v. 36, n. 11, p. 17-19, 1982.
PARRELLA, M. P. Biology of Liriomyza. Annual Review of Entomology, New York, v. 32, n. 76, p. 201–224, 1987.
POSADA, D. Jmodeltest: Phylogenetic Model Averaging. Molecular Biology and Evolution, New York, v. 25, n. 7, p. 1253–1256, 2008.
PUILLANDRE, N. et al. Genetic bottleneck in invasive species: the potato tuber moth adds to the list. Biological Invasions, Netherlands, v. 10, n. 3, p. 319–333, 2008.
REITZ, S. R; GAO, Y.; LEI, Z. Insecticide Use and the Ecology of Invasive Liriomyza Leafminer Management. 1. ed. Rijeka, Croatia: INTECH, 2013. 233 p.
ROZAS, J. et al. DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics, London, v. 19, n. 18, p. 2496–2497, 2003.
SAITOU, N.; NEI, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, New York, v. 4, n. 4, p. 406–425, 1987.
SCHEFFER, S. J.; LEWIS, M. L. Mitochondrial phylogeography of vegetable pest Liriomyza sativae (Diptera: Agromyzidae): divergent clades and invasive populations. Annals of the Entomological Society of America, New York, v. 98, n. 2, p. 181–186, 2005.
SCHEFFER, S. J.; MATTHEW, L. L.; RAVINDRA, C. J. DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Annals of the Entomological Society of America, New York, v. 99, n. 2, p. 204–210, 2006.
SPENCER, K. A. Agromyzidae (Diptera) of Economic Importance. Netherlands. Series Entomologica. 1973. p. 418.
TAJIMA, F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, New York, v. 123, n. 3, p. 585–595, 1989.
TAMURA, K. et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution, New York, v. 28, n. 10, p. 2731–2739, 2011.
TOKUMARU, S. et al. Insecticide susceptibility of Liriomyza sativae, L. trifolii, and L. bryoniae (Diptera: Agromyzidae). Journal of Applied Entomology and Zoology, Tokyo, v. 49, n. 1, p. 1–10, 2005.
WAN, N. F. et al. An ecological indicator to evaluate the effect of chemical insecticide pollution management on complex ecosystems. Ecological Indicators, New York, v. 53, n. 6, p. 11–17, 2015.
WANG, L. P. et al. Genetic variation of host populations of Liriomyza sativae Blanchard. Agricultural Sciences in China, Beijing, v. 7, n. 5, p. 585–590, 2008.
WIEGMANN, B. M. et al. Episodic radiations in the fly tree of life. Proceedings of the National Academy of Sciences, London, v. 108, n. 14, p. 5690-5695, 2011.
WILSON, A. C. et al. Mitochondrial DNA and two perspectives on evolutionary genetics. Biological Journal of the Linnean Society, London, v. 26, n. 1, p. 375–400, 1985.
ALTSCHUL, S. F. et al. "Basic local alignment search tool". Journal of Molecular Biology, London, v. 215, n. 3, p. 403-410, 1990.
BANDELT, H. J.; ROHL, F. P. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, London, v. 16, n. 1, p. 37-48, 1999.
BLACKET, M. J. et al. DNA-based identifications reveal multiple introductions of the vegetable leafminer Liriomyza sativae (Diptera: Agromyzidae) into the Torres Strait Islands and Papua New Guinea. Bulletin of Entomological Research, London, v. 105, n. 5, p. 533-544, 2015.
BUSVINE, J. R. Cryptic species of insect disease vectors and their importance. Endeavour, London, v. 4, n. 3, p. 108-112, 1980.
CLARK, A. R. et al. Invasive phytophagous pests arising through a recent tropical evolutionary radiation: the Bactrocera dorsalis complex of fruit Flies. Annual Review of Entomology, New York, v. 50, n. 1, p. 293-319, 2005.
COSTA-JUNIOR, C. R. L. et al. Genetic structuring and fixed polymorphisms in the gene period among natural populations of Lutzomyia longipalpis in Brazil. Parasites and Vectors, London, v. 8, n. 1, p. 193-202, 2015.
DE SOUZA FREITAS, M. T. et al. Phenotypic and genotypic variations among three allopatric populations of Lutzomyia umbratilis, main vector of Leishmania guyanensis. Parasites and Vectors, London, v. 8, n. 4, p. 448-458, 2015.
DELLA TORRE, A. et al. Molecular and ecological aspects of incipient speciation within Anopheles gambiae: the glass is half full. Environmental Science, Sofia, v. 289, n. 2, p. 115-117, 2002.
EDGAR, R. C. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic acids resources, London, v. 32, n. 5, p. 1792-1797, 2004.
EWING, B. et al. Base-calling of automated sequencer traces using Phred. Genome research, New York, v. 8, n. 3, p. 175-185, 1998.
EXCOFFIER, L.; LISCHER H. E. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology resources, London, v. 10, n. 3, p. 564-567, 2010.
FERGUSON, J. S. Development and stability of insecticide resistance in the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to cyromazine, abamectin, and spinosad. Journal of Economic Entomology, London, v. 97, n. 1, p. 112-119, 2004.
GUINDON, S. et al. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic biology, London, v. 59, n. 3, p. 307–321, 2010.
GARIEPY, T. D. et al. Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods. Journal of applied entomology, Germany, v. 131, n. 4, p. 225–240, 2007.
HEBERT, P. D. N. et al. Biological identifications through DNA barcodes. Proceedings. Biological sciences / The Royal Society, London, v. 270, n. 1512, p. 313-321, 2003.
JENKINS, T. A.; CHAPMAN, J. L.; MICALLEF, O. L. Molecular techniques for the detection and differentiation of host and parasitoid species and the implications for fruit fly management. Journal of Insects, New York, v. 3, n. 3, p. 763–788, 2012.
KIMURA, M. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, Germany, v. 16, n. 2 , p. 111–120, 1980.
KOX, L. F. F. et al. Identification of economically important Liriomyza species by PCR-RFLP analysis. Bulletin OEPP/EPPO, Paris, v. 35, n. 1, p. 79-85, 2005.
LIMA, T. C. C.; GEREMIAS, L. D.; PARRA, J. R. P. Efeito da temperatura e umidade relativa do ar no desenvolvimento de Liriomyza sativae Blanchard (Diptera: Agromyzidae) em Vigna unguiculata. Neotropical Entomology, Piracicaba, v. 38, n. 6, p. 727–733, 2009.
MURPHY, S. T.; LASALLE, J. Review article: balancing biological control strategies in the IPM of new world invasive Liriomyza leafminers in field vegetable crops. Biocontrol News and Information, New York, v. 20, n. 3, p. 91-104, 1999.
NAKANO, O.; WIENDL, F. M.; MINAMI, K. Uma nova praga (Agromyzidae) da couve. Revista Agrícola, Piracicaba, v. 42, n. 1, p. 1-10, 1967.
NEI, M. Bottlenecks, genetic polymorphism and speciation. Genetics, New York, v. 170, n. 1, p. 1-4, 2005.
NELSON, L. A.; WALLMAN J. F.; DOWTON, M. Using COI barcodes to identify forensically and medically important blowflies. Medical and Veterinary Entomology, London, v. 21, n. 1, p. 44-52, 2007.
PANG, B.P. et al. Effects of different host plants on population parameters of Liriomyza sativae. Plant Protection, London, v. 31, n. 2, p. 26-28, 2005.
PARRELLA, M. P. et al. Control of Liriomyza trifolii with biological agents and insects growth regulators. California Agriculture, Richmond, v. 36, n. 11, p. 17-19, 1982.
PARRELLA, M. P. Biology of Liriomyza. Annual Review of Entomology, New York, v. 32, n. 76, p. 201–224, 1987.
POSADA, D. Jmodeltest: Phylogenetic Model Averaging. Molecular Biology and Evolution, New York, v. 25, n. 7, p. 1253–1256, 2008.
PUILLANDRE, N. et al. Genetic bottleneck in invasive species: the potato tuber moth adds to the list. Biological Invasions, Netherlands, v. 10, n. 3, p. 319–333, 2008.
REITZ, S. R; GAO, Y.; LEI, Z. Insecticide Use and the Ecology of Invasive Liriomyza Leafminer Management. 1. ed. Rijeka, Croatia: INTECH, 2013. 233 p.
ROZAS, J. et al. DnaSP, DNA polymorphism analysis by the coalescent and other methods. Bioinformatics, London, v. 19, n. 18, p. 2496–2497, 2003.
SAITOU, N.; NEI, M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, New York, v. 4, n. 4, p. 406–425, 1987.
SCHEFFER, S. J.; LEWIS, M. L. Mitochondrial phylogeography of vegetable pest Liriomyza sativae (Diptera: Agromyzidae): divergent clades and invasive populations. Annals of the Entomological Society of America, New York, v. 98, n. 2, p. 181–186, 2005.
SCHEFFER, S. J.; MATTHEW, L. L.; RAVINDRA, C. J. DNA barcoding applied to invasive leafminers (Diptera: Agromyzidae) in the Philippines. Annals of the Entomological Society of America, New York, v. 99, n. 2, p. 204–210, 2006.
SPENCER, K. A. Agromyzidae (Diptera) of Economic Importance. Netherlands. Series Entomologica. 1973. p. 418.
TAJIMA, F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, New York, v. 123, n. 3, p. 585–595, 1989.
TAMURA, K. et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology and Evolution, New York, v. 28, n. 10, p. 2731–2739, 2011.
TOKUMARU, S. et al. Insecticide susceptibility of Liriomyza sativae, L. trifolii, and L. bryoniae (Diptera: Agromyzidae). Journal of Applied Entomology and Zoology, Tokyo, v. 49, n. 1, p. 1–10, 2005.
WAN, N. F. et al. An ecological indicator to evaluate the effect of chemical insecticide pollution management on complex ecosystems. Ecological Indicators, New York, v. 53, n. 6, p. 11–17, 2015.
WANG, L. P. et al. Genetic variation of host populations of Liriomyza sativae Blanchard. Agricultural Sciences in China, Beijing, v. 7, n. 5, p. 585–590, 2008.
WIEGMANN, B. M. et al. Episodic radiations in the fly tree of life. Proceedings of the National Academy of Sciences, London, v. 108, n. 14, p. 5690-5695, 2011.
WILSON, A. C. et al. Mitochondrial DNA and two perspectives on evolutionary genetics. Biological Journal of the Linnean Society, London, v. 26, n. 1, p. 375–400, 1985.
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