TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

Authors

  • Antônia Adailha Torres Souza Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0003-3934-8912
  • Narjara Walessa Nogueira Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0003-0668-3327
  • Salvador Barros Torres Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0003-0668-3327
  • Clarisse Pereira Benedito Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0002-2846-1162
  • Maria Lilia de Souza Neta Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0002-2108-7526
  • Emanoela Pereira de Paiva Center of Agricultural Sciences, Universidade Federal Rural do Semi-Árido, Mossoró, RN http://orcid.org/0000-0003-4510-9205

DOI:

https://doi.org/10.1590/1983-21252018v31n403rc

Keywords:

Malvaceae. Biochemical test. Quick tests.

Abstract

This study aimed to establish an adequate tetrazolium test procedure to estimate okra seed viability. Initially, seeds from four okra lots were immersed in alcohol (98%) for 60 minutes, followed by an  18-h soak in distilled water at 25 °C. Then, the seeds were longitudinally sectioned, immersed in 0.025, 0.050 and 0.075% tetrazolium solutions for 60, 90 and 120 minutes and maintained in an oven at 35 and 40 °C. Subsequently, the seeds were evaluated for viability, based on tissue color (bright red, pink or dark Carmine red). Simultaneously, a germination test was conducted, and its results were used as a reference for the tetrazolium test. The experiment was carried out in a completely randomized design, in a 3 x 3 + 1 factorial scheme (three concentrations of tetrazolium salt x three immersion periods, plus the control) in four replicates of 50 seeds for each treatment. The tetrazolium test to estimate okra seed viability should be conducted in 0.075% tetrazolium solution, for 90 minutes, at 35 °C.

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Published

16-10-2018

Issue

Section

Agronomy