QUALITY, ANTIOXIDANT AND ENZYMATIC ACTIVITIES OF FACHEIRO (Pilosocereus pachycladus RITTER) FRUITS DURING MATURATION
DOI:
https://doi.org/10.1590/1983-21252019v32n426rcKeywords:
Cactaceae. Maturity. Bioactive Compounds. DPPH. Peroxidase activity.Abstract
The fruit of Cactaceas from northeastern Brazil have been the object of many ethnobotanical and functional quality studies. However, a considerable number of species remain poorly exploited, such as the facheiro (Pilosocereus pachycladus Ritter), a native Brazilian plant widely occurring and used in the Caatinga. The objectives of this research were to evaluate the quality, and antioxidant and enzymatic activities of the facheiro fruit during maturation. Fruits were harvested from areas of occurrence of plants in the Paraíba State, Brazil, at three maturity stages, which were determined based on the color of the epicarp (G - green, IP – green with purple traces, and R - completely purple). It was carried out a survey of terms sensorial descriptor terms for fruit appearance and then evaluated the physical, physicochemical, bioactive compounds, antioxidant and peroxidase (POD) activities during maturation. The fruit were described as fleshy berries, with a smooth epicarp; a round, flat shape; opaque purple color; and juicy and soft pulp when ripe. Quality varied during maturation, with the most significant changes being in coloration: the fruit became more purple over time, providing a clear indicator of maturity. With maturation, there was an increase in the content of betalains and total extractable polyphenols, as well as antioxidant activity, by DPPH radical capture method, mainly in the pulp. The pulp of the Pilosocereus pachycladus fruit is an important source of betalains, primarily betacyanins. During maturation, POD activity decreased in the pericarp and increased in the pulp and was strongly correlated with the presence of betalains and with antioxidant activity.
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References
ABUD, H. F. et al. Germination and morphological characterization of the fruits, seeds, and seedlings of Pilosocereus gounellei. Brazilian Journal of Botany, v. 35, n. 1, p. 11-16, 2012.
ABUD, H. F. et al. Germinação, expressão morfológica de frutos, sementes e plântulas de Pilosereus pachycladus Ritter. Revista Ciência Agronômica, v. 41, n. 3, p. 468-474, 2010.
AZERÊDO, L. P. M. et al. Qualidade de manga ‘Tommy Atkins’ da produção integrada recoberta com fécula de mandioca associada a óleos essenciais e quitosana. Revista Brasileira de Fruticultura, v. 38, n. 1, p. 141-150, 2016.
BRADFORD, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, v. 72, n. 1-2, p. 248-254, 1976.
DAMODARAN, S.; PARKIN, K. L. Química de Alimentos de Fennema. 5. ed. Porto Alegre, RS: Artmed, 2019. 1112 p.
DANTAS, R. L. et al. Quality and descriptive terminology of Tacinga inamoena (K. Schum.) fruits. Acta Horticulture, v. 1067, n. 8, p. 143-149, 2015a .
DANTAS, R. L. et al. Changes during maturation in the bioactive compounds and antioxidant activity of Opuntia stricta (HAW.) fruits. Acta Horticulture, v. 1067, n. 8, p. 159-165, 2015b.
DANTAS, R. L. et al. Betalains and Antioxidant Activity in Fruits of Cactaceae from Brazilian Semiarid. Acta Horticulture, v. 1067, n. 8, p. 151-157, 2015c.
DANTAS, R. L. et al. Bioactive compounds and antioxidant activity of Tacinga inamoena (K. Schum.) [NP Taylor &Stuppy] fruit during maturation. African Journal of Agricultural, v. 11, n. 434, p. 1511–1518, 2016.
DUARTE, L. E. B.; RIVERA, J. A. C.; CUENCA, C. E. N. Catalasa, peroxidasa y polifenoloxidasa en pitaya amarilla (Acanthocereus pitajaya): maduración y senescencia. Acta Biológica Colombiana, v. 10, n. 2. p. 49-60, 2005.
FRANCIS, F. J. Analysis of Anthocyanins. In: MARKAKIS, P. (Ed.). Anthocyanins As Food Colors. Lodon, UK: Academic Press Inc., 1982. cap. 7, p. 181-207.
INSTITUTO ADOLFO LUTZ - IAL. Normas Analíticas do Instituto Adolfo Lutz: métodos químicos e físicos para análise de alimentos. 5. ed. São Paulo, SP: Instituto Adolfo Lutz, 2005. 371 p.
KOHAICH, K.; BAAZIZ, M. New investigations on the guaiacol peroxidase of Opuntia ficus indica L. and its modulation by ascorbic acid and copper. Towards an optimization of quantitative and qualitative tests. Journal of Molecular Catalysis B: Enzymatic, v. 119, n. 9, p. 26-32, 2015.
LARRAURI, J. A.; RUPÉREZ, P.; SAURA-CALIXTO, F. Effect drying temperature on the stability of polyphenols and antioxidant activity of red grape pomace peels. Journal Agricultural and Food Chemistry, v. 45, n. 4, p. 1390-1393, 1997.
LEAL, I. R.; WIRTH, R.; TABARELLI, M. Seed dispersal by ants in the semi-arid Caatinga of northeast Brazil. Annals of Botany, v. 99, n. 5, p. 885-894, 2007.
LIMA, C. A. et al. Características físico-químicas, polifenóis e flavonoides amarelos em frutos de espécies de pitaias comerciais e nativas do cerrado. Revista Brasileira de Fruticultura, v. 35, n. 2, p. 565-570, 2013.
MEDEIROS, R. L. S. et al. Germinação e vigor de sementes de Pilosocereus catingicola (Gürke) Byles e Rowleysubsup. Salvadorensis (Werderm.) Zappi (Cactaceae) da Caatinga paraibana. Gaia Scientia, v. 9, n. 2, p. 61-66, 2015.
MELO, R. S. et al. Maturação e qualidade de frutos de mandacaru (Cereus jamacaru P.DC.) de diferentes bioclimas do estado da Paraíba. Revista Agropecuária Técnica, v. 38, n. 3, p. 160-168, 2017.
SATO, S. T. A. et al. Caracterização física e físico-química de pitayas vermelhas (Hylocereus costaricensis) produzidas em três municípios paraenses. Journal of Bioenergy and Food Science, v. 1, n. 2, p. 16-46, 2014.
SIDDIQ, M. W. Preharvest modulation of postharvest fruit and vegetable quality. 1. ed. London, UK: Academic Press, 2018. 498 p.
SIEGEL, B. Z. Plant peroxidases – an organismic perspective. Plant Growth Regulation, v. 12, n. 3, p. 303-312, 1993.
STONE, H.; SIDEL, J. L. Quantitative descriptive analysis: developments, application, and the future. Food Technology, v. 52, n. 8, p. 48-52, 1998.
SILVA, A. A. et al. Physical and physicochemical characteristics, bioactive compounds and antioxidant activity of xiquexique (Pilosocereus gounellei (A. Weber ex K. Schum,)) fruit. Semina: Ciências Agrárias, v. 39, n. 5, p. 1969-1980, 2018.
SILVA, S. M. et al. Features of postharvest physiology and quality of Cactaceae fruits from Brazilian Northeast. Acta Horticulture, v. 811, n. 6, p. 113-122, 2009.
SOUZA, R. L. A. et al. Physicochemical, bioactive and functional evaluation of the exotic fruits Opuntia ficus-indica and Pilosocereus pachycladus Ritter from the Brazilian caatinga. Journal of Food Science and Technology, v. 52, n. 11, p. 7329-7336. 2015.
STINTZING, F. et al. Color, betalain pattern and antioxidant properties of Cactus pear (Opuntia spp) clones. Journal of Agriculture and Food Chemistry, v. 53, n. 2, p. 442-451, 2005.
STROHECKER, R.; HENNING, H. M. Analisis de vitaminas: métodos comprobados. Madrid, ES: Paz Montalvo, 1967. 428 p.
TALEMI, E. P.; SEDAGHATHOOR, S. A study on biochemical properties of different parts of three Opuntia species (Opuntia ficus-indica, O. microdasys, and O.basilaris). Journal of the Professional Association for Cactus Development, v. 19, n. 1, p. 32-44, 2017.
TESORIERE, L. et al. Indicaxanthin inhibits NADPH oxidase (NOX)-1 activation and NF-kB-dependent release of inflammatory mediators and prevents the increase of epithelial permeability in IL-1b-explosed Caco-2 cells. British Journal of Nutrition, v. 111, n. 3, p. 415-423, 2014.
TORRES, L. B. V. et al. Changes in color and bioactive components during maturation of cactus pear fruits from Curimataú micro-region, Paraiba state, Brazil. Acta Horticulturae, v. 811, n. 6, p. 137-141, 2009.
WU, G. L. et al. Fluroxypyr triggers oxidative damage by producing superoxide and hydrogen peroxide in rice (Oryza sativa). Ecotoxicology, v. 19, n. 1, p. 124-132, 2010.
YAHIA, E. M.; JACOBO, C. M. Nutritional components and anti-oxidant capacity of ten cultivars and lines of cactus pear fruit (Opuntia spp.). Food Research International, v. 44, n. 7, p. 2311-2318, 2011.
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