ENTOMOPATHOGENIC NEMATODES ON CONTROL OF MEDITERRANEAN FRUIT FLY (DIPTERA: TEPHRITIDAE)
DOI:
https://doi.org/10.1590/1983-21252020v33n412rcKeywords:
Ceratitis capitata. Steinernema carpocapsae. Heterorhabditis amazonensis. Biological control.Abstract
Mediterranean fruit fly (Ceratitis capitata) is one of the world's major pests infesting fruit crops, causing significant losses. However, this insect has a soil phase and is a potential target for entomopathogenic nematodes. The objective of this work was to test the efficiency of the nematodes Steinernema carpocapsae ALL and Heterorhabditis amazonensis JPM4 in controlling larvae and pupae of Ceratitis capitata. Petri dishes with filter paper or pots with 200 g of soil were used to assess different nematode concentrations; 20 individuals (larvae or pupae) were placed in each container and the nematode suspension was applied at concentrations of 0, 140, 180, 220, 260, and 300 IJs (infective juveniles) cm-2. The nematode horizontal movement was tested using pots with soil and a metal screen at every 10 cm to define different distances (0, 10, 20, and 30 cm from the application site). Ten larvae and ten pupae were transferred to every distance; 10 mL of the nematode suspension (220 IJs cm-2) were applied at the distance zero; 10 mL of the nematode suspension with 220 IJs cm-2 was applied to each pot, using ten larvae and ten pupae for each evaluation time (0, 10, 20, and 30 days after application of nematodes) to study the effectiveness of these nematodes over time. Both nematodes killed C. capitata larvae and pupae, but S. carpocapsae ALL was the most effective. However, this nematode showed no ability for horizontal movement, which was found in H. amazonensis JPM4 nematodes. Both nematodes showed decreasing efficiency over time.
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References
ADAMS, B. J.; NGUYEN, K. B. Taxonomy and systematics. In: GAUGLER, R. (Ed). Entomopathogenic nematology. Wallingford, Oxon: CABI Publishing, 2002. cap. 1, p. 1-28.
ANDALÓ, V. et al. Movement of Heterorhabditis amazonesis and Steinernema arenarium in search of corn fall armyworm larvae in artificial conditions. Scientia Agricola, 3: 226-230, 2012.
ANDALÓ, V.; MOREIRA, G. F.; MOINO Jr, A. Studies of two new populations of Heterorhabditis amazonensis (Rhabditida: Heterorhabditidae). Nematropica, 39: 199-211, 2009.
ANDALÓ, V.; MOREIRA, G. F.; MOINO Jr, A. Heterorhabditis amazonensis RSC5 (Rhabditida: Heterorhabditidae) movement and host recognition. Revista Colombiana de Entomologia, 40: 91-97, 2014.
CHERGUI, S. et al. Efficacy of Turkish isolate of Steinernema feltiae (Rhabditida: Steinernematidae) in controlling the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), under laboratory conditions. Egyptian Journal of Biological Pest Control, 29: 01-07, 2019.
DIAS, N. P. et al. Fruit fly management research: A systematic review of monitoring and control tactics in the world. Crop Protection, 12:187:200, 2018.
EGARTNER, A. et al. Recent records of the Mediterranean fruit fly, Ceratitis capitata (Tephritidae, Diptera), in Austria. IOBC-WPRS Bulletin, 146: 143-152, 2019.
EL-SADAWY, H. A. Effect of temperature and soil moisture on the infectivity of some entomopathogenic nematodes against larvae of the rice moth and flesh fly. International Journal of Nematology, 11: 58-62, 2001.
FOELKEL, E.; MONTEIRO, L. B.; VOSS, M. Virulence of nematodes against larvae of the south-American fruit fly in laboratory using soil from Porto Amazonas, Paraná, Brazil, as substrate. Ciência Rural, 46: 405-410, 2016.
GAUGLER, R.; WANG, Y.; CAMPBELL, J. F. Aggressive and evasive behaviors in Popillia japonica (Coleoptera: Scarabaeidae) larvae: defences against entomopathogenic nematode attack. Journal of Invertebrate Pathology, 64: 193-199, 1994.
GODJO, A. et al. Pathogenicity of indigenous entomopathogenic nematodes from Benin against mango fruit fly (Bactrocera dorsalis) under laboratory conditions. Biological Control, 117: 68-77, 2018.
GRIFFIN, C. et al. Biology and behaviour. In: GREVAL, P., EHLERS, R. U., SHAPIRO-ILAN, D. (Eds.). Nematodes as Biocontrol Agents. Wallingford, Oxon: CABI Publishing, 2005. cap 2, p. 47–64.
HEVE, W. K. et al. Biological control potential of entomopathogenic nematodes for management of caribbean fruit fly, Anastrepha suspensa Loew (Tephritidae). Pest Management Science, 73: 1220–1228, 2017.
KEPENEKCI, I.; HAZIR, S.; ÖZDEM, A. Evaluation of native entomopathogenic nematodes for the control of the erupean cherry fruit fly Rhagoletis cerasi L. (Diptera: Tephritidae) larvae in soil. Turkish Journal of Agriculture and Forestry, 39: 74-79, 2015.
KHOURY, Y. E. et al. Effect of temperature on the pathogenicity of mediterranean native entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from natural ecosystems. Redia, 101: 123-127, 2018.
LEWIS, E. E. et al. Behavioral ecology of entomopathogenic nematodes. Biological Control, 38: 66-79, 2006.
MALAN, A. P.; MANRAKHAN, A. Susceptibility of the mediterranean fruit fly (Ceratitis capitata) and the natal fruit fly (Ceratitis rosa) to entomopathogenic nematodes. Journal of Invertebrate Pathology, 100: 47-49, 2009.
JAMES, M.; MALAN, A. P.; ADDISON, P. Surveying and screening south african entomopathogenic nematodes for the control of the mediterranean fruit fly, Ceratitis capitata (Wiedemann). Crop Protection, 105: 41-48, 2018.
LABAUDE, S.; GRIFFIN, C. Transmission success of entomopathogenic nematodes used in pest control. Insects, 72: 1-20, 2018.
MASTORE, M. et al. Susceptibility to entomopathogens and modulation of basal immunity in two insect models at different temperatures. Journal of Thermal Biology, 79: 15-23, 2019.
MINAS, R. S. et al. Potential of entomopathogenic nematodes (Rhabditida: Heterorhabditidae) to control Mediterranean fruit fly (Diptera: Tephritidae) soil stages. Nematoda, 3: 1-14, 2016.
MORTON, A.; GARCÍA-DEL-PINO, F. Ecological characterization of entomopathogenic nematodes isolated in stone fruit orchard soils of mediterranean areas. Journal of Invertebrate Pathology, 102: 203-213, 2009.
MURIITHI, B. W. et al. Impact assessment of Integrated Pest Management (IPM) strategy forsuppression of mango-infesting fruitflies in Kenya. Crop Protection, 81: 20-29, 2016.
ROHDE, C. et al. Influence of soil temperature and moisture on the infectivity of entomopathogenic nematodes (Rhabditida: Heterorhabiditidae e Steinernematidae) against larvae of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Neotropical Entomology, 39: 608-611, 2010.
ROHDE, C. et al. Selection of entomopathogenic nematodes for the controlo of the fruit fly Ceratitis capitata (Diptera: Tephritidae). Revista Brasileira de Ciências Agrárias, 7: 797-802, 2012.
ROHDE, C. et al. Compatibilidade de nematóides entomopatogênicos e extratos vegetais aquosos visando o controle da mosca-das-frutas Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Semina, 34: 1033-1042, 2013.
SELVAN, S.; CAMPBELL, J. F.; GAUGLER, R. Density-dependent effects on entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) within an insect host. Journal of Invertebrate Pathology, 62: 278-284, 1993.
SHAPIRO-ILAN, D. I; HAN, R.; DOLINKI, C. Produção de nematóides entomopatogênicos e tecnologia de aplicação. The Journal of Nematology, 44: 206 - 217, 2012.
SHAPIRO-ILAN, D. I., HAZIR, S., LEITE, L. Viability and virulence of entomopathogenic nematodes exposed to ultraviolet radiation. Journal of Nematology, 47:184-189, 2015.
SHAPIRO-ILAN, D. I.; HAZIR, S.; GLAZER, I. Basic and applied research: entomopathogenic nematodes. In: LACEY, L. A. (Ed.). Microbial control of insect and mite pests: from theory to practice. London: Academic Press, 2017. cap. 6, p. 91-105.
SIRJANI, F. O.; LEWIS, E. E.; KAYA, H. K. Evaluation of entomopathogenic nematodes against the olive fruit fly Bactrocera oleae (Diptera: Tephritidae). Biological Control, 38: 124-133, 2009.
TORRINI, G., et al. Entomopathogenic nematodes as potential biocontrol agents against Bactrocera oleae (Diptera: Tephritidae). Biocontrol Science Technology, 30:1-12, 2020.
ULU, T. C., SUSURLUK, I. A. Heat and desiccation tolerances of Heterorhabditis bacteriophora strains and relationships between their tolerances and some bio-ecological characteristics. Invertebrate Survival Journal, 11: 4-10, 2014.
WOODRING, J. L.; KAYA, H. K. Steinernematidae and Heterorhabditidae nematodes: a handbook of techniques. Nemathode Subcommittee on the Southern Regional Project S135 - Entomopathogens for use in Pest Management Systems, Arkansas: Southern Cooperative, 1988. (Series Bulletin, 331).
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