APLICAÇÕES DE MARCADORES ISSR EM ESTUDOS DE DIVERSIDADE GENÉTICA DE Pityrocarpa moniliformis

Francival Cardoso Felix; Kyvia Pontes Teixeira das Chagas; Cibele dos Santos Ferrari; Fábio de Almeida Vieira; Mauro Vasconcelos Pacheco

doi:10.1590/1983-21252020v33n417rc

Authors

Francival Cardoso Felix Specialized Academic Unit in Agricultural Sciences, Universidade Federal do Rio Grande do Norte, Macaíba, RN https://orcid.org/0000-0002-6518-5697
Kyvia Pontes Teixeira das Chagas Specialized Academic Unit in Agricultural Sciences, Universidade Federal do Rio Grande do Norte, Macaíba, RN https://orcid.org/0000-0003-1361-3204
Cibele dos Santos Ferrari Specialized Academic Unit in Agricultural Sciences, Universidade Federal do Rio Grande do Norte, Macaíba, RN https://orcid.org/0000-0003-2940-0190
Fábio de Almeida Vieira Specialized Academic Unit in Agricultural Sciences, Universidade Federal do Rio Grande do Norte, Macaíba, RN https://orcid.org/0000-0003-3347-255X
Mauro Vasconcelos Pacheco Specialized Academic Unit in Agricultural Sciences, Universidade Federal do Rio Grande do Norte, Macaíba, RN https://orcid.org/0000-0002-0447-9800

DOI:

https://doi.org/10.1590/1983-21252020v33n417rc

Keywords:

Semiarid. Conservation. Dry forests. Inter-Simple Sequence Repeat. Molecular markers.

Abstract

Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (Fabaceae) is a native brazilian species with high potential for economic development programs in semiarid regions, mainly related to the production of honey, animal food and firewood. Thus, the objective of this work was to select Inter-Simple Sequence Repeat (ISSR) molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population. For this, 28 ISSR molecular markers were tested, evaluating the total number of loci, polymorphism rate and the Polymorphism Information Content (PIC) for the selected primers, the “Marker Index”, and the “Resolving Power”. Genetic diversity parameters (Nei genetic distance and Shannon index) were evaluated for 30 individuals located in Macaíba, Rio Grande do Norte State, Brazil. Seven primers were selected, which provided 74 loci, with 82% being polymorphic, while the PIC value was 0.344. The Nei genetic distance was 0.244, and the Shannon index was 0.374. Therefore, ISSR molecular markers (UBC 827, 840, 844, 857, 859, 860 and 873) are considered efficient in studying the genetic diversity of populations for the selection of matrices and germplasm banks, and may contribute to the conservation and genetic improvement of P. moniliformis populations.

Downloads

Download data is not yet available.

References

ALVES, M. J. et al. Phenols, flavonoids and antioxidant and cytotoxic activity of leaves, fruits, peel of fruits and seeds of Piptadenia moniliformis Benth (Leguminosae – Mimosoideae). Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, 13: 466-476, 2014.

ANDERSON, J. A. et al. Optimizing parental selection for genetic linkage maps. Genome, 36: 181-186, 1993.

ARAÚJO, F. S. et al. ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth. Idesia, 34: 47-52, 2016.

AZERÊDO, G. A.; PAULA, R. C.; VALERI, S. V. Determining the viability of Piptadenia moniliformis Benth seeds with the tetrazolium test. Journal of Seed Science, 33: 61-68, 2011.

BALLESTA, P. et al. Analysis of the genetic diversity of Eucalyptus cladocalyx (sugar gum) using ISSR markers. Acta Scientiarum - Agronomy, 37: 133-140, 2015.

BOTSTEIN, D. et al. Construction of genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, 32: 314-331, 1980.

CHAGAS, K. P. T. et al. Genetic diversity of long-established populations of Elaeis guineensis Jacquin (Arecaceae). Revista Brasileira de Fruticultura, 41: e023, 2019.

CHEN, Y. et al. Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers. Plos One, 12: 01-14, 2017.

COSTA, D. F. et al. Genetic diversity and selection of ISSR primers in a natural mangaba population (Hancornia speciosa Gomes) (Apocynaceae). Revista Brasileira de Fruticultura, 37: 970-976, 2015.

COSTA, J. C. et al. Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers. Genetics and Molecular Research, 17: 1-8, 2018.

COSTA, T. S. et al. Genetic diversity of accessions of the mangaba germplasm bank in Sergipe, Brazil. Pesquisa Agropecuária Brasileira, 46: 499-508, 2011.

DOYLE, J. J.; DOYLE, J. L. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15, 1987.

DUARTE, M. M.; NOGUEIRA, A. C.; VIEIRA, E. S. N. Diversity and spatial genetic structure of natural populations of Ziziphus joazeiro Mart. Brazilian Journal of Agricultural Sciences, 13: e5573, 2018.

FARIAS, D. F. et al. Water extracts of brazilian leguminous seeds as rich sources of larvicidal compounds against Aedes aegypti L. Anais da Academia Brasileira de Ciências, 82: 585-594, 2010.

FAJARDO, C. G. et al. Genetic and phenotypic association of the carnauba palm tree evaluated by inter-simple sequence repeat and biometric traits. Genetics and Molecular Research, 17: 1-9, 2018.

GONÇALVES, L. O. et al. Caracterização genética de mulungu (Erythrina velutina Willd.) em áreas de baixa ocorrência. Revista de Ciência Agronômica, 45: 290-298, 2014.

GOOGLE EARTH PRO. Google Earth Pro para PC, Mac ou Linux. Disponível em: <https://www.google.com.br/earth/download/gep/agree.html>. Acesso em: 01 jul. 2018.

JESUS, M. C. et al. Study of pollen from light honeys produced in Piauí State, Brazil. Palynology, 39: 110-124, 2015.

KOUR, B. et al. In vitro mass multiplication and assessment of genetic stability of in vitro raised Artemisia absinthium L. plants using ISSR and SSAP molecular markers, Advances in Botany, 2014: 01-07, 2014.

KRUSKAL, J. B. Multidimensional scaling by optimizing goodness of fit to a no metric hypothesis. Psychometrika, 29: 1-27, 1964.

LOPES, J. S.; COSTA, M. R. J.; ARRIEL, D. A. A. Genetic diversity of potential mother trees of Myracrodruon urundeuva Allemão in a remnant population from Brazilian Cerrado using ISSR. Advances in Forestry Science, 7: 1017-1024, 2020.

LORENZONI, R. M. et al. Using ISSR for finger printing accessions of biribazeiro. Revista Brasileira de Fruticultura, 36: 251-257, 2014.

NASCIMENTO, M. F. et al. OSB panels made with wood species from the Brazilian Northeast's Caatinga. Ambiente Construído, 15: 41-48, 2015.

NEVES, A. G. S. et al. Selection of ISSR molecular primers for genetic variability studies of Syagrus cearensis Noblick. Agropecuária Científica no Semiárido, 15: 228-231, 2019.

NING, Z. et al. Core germplasm construction of Cornus officinalis by ISSR markers. Journal of Agricultural Biotechnology, 2: 579-587, 2017.

NYBOM, H. Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology, 13: 1143-1155, 2004.

PREVOST, A.; WILKINSON, M. J. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112, 1999.

ROSA, J. D. et al. Variability and population genetic structure in Achyrocline flaccida (Weinm.) DC., a species with high value in folk medicine in South America. Plos One, 12: e0183533, 2017.

SILVA, T. S. S. et al. Caracterização e seleção de marcadores moleculares em Croton linearifolius Mull. Arg. como subsídio para estudos genéticos. Multi-Science Journal, 1: 04-08, 2018.

SIMON, M. F. Manual de curadores de germoplasma - vegetal: conservação in situ. Brasília: Embrapa Recursos Genéticos e Biotecnologia, Colombo, 322: 1-14, 2010.

VARSHNEY, R. K. et al. Comparative assessment of ESTSSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Science, 173: 628-649, 2007.