MODULAÇÃO DO MEIO DE CULTURA NA CONSERVAÇÃO EX SITU DE Neoregelia mucugensis Leme (BROMELIACEAE)

Andressa Priscila Piancó Santos Lima; Fernanda de Jesus Oliveira Bastos; Alone Lima-Brito; Gilênio Borges Fernandes; José Raniere Ferreira de Santana

doi:10.1590/1983-21252021v34n403rc

Authors

Andressa Priscila Piancó Santos Lima Department of Biological Sciences, Universidade Estadual de Feira de Santana, BA https://orcid.org/0000-0002-8272-3506
Fernanda de Jesus Oliveira Bastos Department of Biological Sciences, Universidade Estadual de Feira de Santana, BA https://orcid.org/0000-0001-7180-6405
Alone Lima-Brito Department of Biological Sciences, Universidade Estadual de Feira de Santana, BA https://orcid.org/0000-0003-3481-6481
Gilênio Borges Fernandes Department of Exacts, Universidade Estadual de Feira de Santana, BA https://orcid.org/0000-0002-2364-0954
José Raniere Ferreira de Santana Department of Biological Sciences, Universidade Estadual de Feira de Santana, BA https://orcid.org/0000-0003-0186-6888

DOI:

https://doi.org/10.1590/1983-21252021v34n403rc

Keywords:

Slow growth. Bromeliad. Osmoregulators.

Abstract

Bromeliads are the target of predatory extractivism and consequently many species are included in the red list of threatened species, such as those belonging to the genus Neoregelia. Although Neoregelia mucugensis has not been evaluated for the degree of threat, its exploitation is exclusively extractive and its occurrence in Chapada Diamantina-BA is subject to the action of fires that affect the region annually. In this context, applying techniques aimed at protecting this genetic resource is fundamental for both the maintenance of its natural populations and the ex situ conservation of this genetic material. Plant tissue culture techniques have been successfully applied for the conservation of several bromeliad species. One of the methods used is slow growth, which consists in reducing plant metabolism and consequently decelerating its growth, which allows the maintenance of in vitro plant collections without the need for subculture. In this context, the objective of this study was to test the reduction of salts in the culture medium and the addition of osmoregulators on the induction of slow growth of N. mucugensis. Plants were subjected to treatments composed of different concentrations of MS medium and mannitol for a period of 12 months, when then analyses were conducted to evaluate growth, chlorophyll content and regeneration capacity of shoots in vitro. It was found that the treatment containing MS ½ and 7.8 g.L^-1 of mannitol is indicated for in vitro conservation of N. mucugensis with maintenance of the regenerative capacity of its tissues.

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