Culture of swine cardiomyocites

Authors

  • Caio Sabino de Oliveira
  • Érika Branco
  • Emerson Ticona Fioreto
  • Daniele dos Santos Martins
  • Guilherme Buzon Gregores
  • Rosa Cabral
  • Guilherme José Ferreira
  • Carlos Alberto Palmeira Sarmento
  • Maria Angelica Miglino

DOI:

https://doi.org/10.21708/avb.2009.3.3.1438

Abstract

Cardiac insufficiency is one of the most harmful disease in the occidental world; A range of researches have been developed in order to provide new and more efficient therapeutics for the disease. New therapeutics purposes have been directed to the origin of the pathological process and aim to re-establish functional physiology, as in transplants of cells like smooth or striated muscular cells, fetal or adult cardiomyocytes into the fibroid myocardium. In this study, previous published protocols developed in rats and mice were adapted for swine cardiomyocytes. The main goal was to stablish a viable protocol for swine cardiomiocyte culture, and the establishment of an experimental model using swine. Three protocols were tested (A - enzimatic digestion in adult heart; B- enzimatic digestion in fetal heart; C - explant of samples of adult and fetal hearts. We observed that protocol B produced a reliable result suggesting cardiac fibre formation, although, further immunecitochemistry and ultrastrucutural will be investigated to confirm the hypothesis. Keywords: Cell culture, heart, swine cardiomyocites.

Downloads

Download data is not yet available.

Published

2009-12-24

Issue

Section

Original Articles / Artigos de Pesquisa

Most read articles by the same author(s)

<< < 1 2