Amprolium-induced thiamine deficiency in mice: evaluation of a practical model by oral administration

  • Fabiano Mendes de Cordova Universidade Federal do Tocantins http://orcid.org/0000-0003-4735-4108
  • Clarissa Amorim Silva de Cordova Universidade Federal do Tocantins
  • Rita de Cássia Noronha Medeiros Universidade Federal do Tocantins
  • Juliana Oliveira Moraes Universidade Federal do Tocantins
  • Samara Dias Cardoso Rodrigues Universidade Federal do Tocantins
  • Helen Quézia da Silva Aguiar Universidade Federal do Tocantins
  • Leidiano Martins Pereira Universidade Federal do Tocantins

Resumo

Thiamine is an essential cofactor for several cellular functions. Your deficiency results in important neurological disorders, with mechanisms and lesions not fully understood. The purpose of this work was to evaluate a thiamine deficiency through the model of oral administration of amprolium in mice. The animals, treated for 20 or 80 days, received amprolium in drinking water at doses of 10, 20, and 30 mg/mL (deficient groups A, B, and C, respectively). Deficient groups A and B showed reduction in body weight gain and performance changes in the open field (decreased distance and rearing, and increased grooming) and rotarod (reduced latency to fall) behavioural tests, when treated for 80 days. However, no histological changes were observed in the central nervous system. Moreover, group B animals exposed to amprolium developed proteinuria, with moderate tubular nephrosis, at 80 days. At the highest dose (group C) there was no interest to drink water. The data suggest that the use of oral amprolium in mice may be an interesting and viable model, when using adequate exposure times and doses. The amprolium induces thiamine deficiency progressively and moderately, which may be potentially useful for disturbed pathogenesis studies.

Downloads

Não há dados estatísticos.

Biografia do Autor

Fabiano Mendes de Cordova, Universidade Federal do Tocantins
Curso de Medicina Veterinária Pós-Graduação em Sanidade Animal e Saúde Pública nos Trópicos Setor de Patologia Veterinária
Clarissa Amorim Silva de Cordova, Universidade Federal do Tocantins
Curso de Medicina
Rita de Cássia Noronha Medeiros, Universidade Federal do Tocantins
Pós-Graduação em Sanidade Animal e Saúde Pública nos Trópicos
Juliana Oliveira Moraes, Universidade Federal do Tocantins
Pós-Graduação em Sanidade Animal e Saúde Pública nos Trópicos
Samara Dias Cardoso Rodrigues, Universidade Federal do Tocantins
Curso de Medicina Veterinária
Helen Quézia da Silva Aguiar, Universidade Federal do Tocantins
Curso de Medicina Veterinária Setor de Patologia Veterinária
Leidiano Martins Pereira, Universidade Federal do Tocantins
Curso de Medicina Veterinária
Publicado
2017-10-12
Seção
Original Articles / Artigos de Pesquisa