In vitro efficacy of cyclobutrifluram and biocontrol microorganisms against banana plant-parasitic nematodes

Authors

  • Bruno Santos Louzado das Neves Center of Agrarian, Environmental and Biological Sciences, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil https://orcid.org/0000-0002-3112-1484
  • Liliane Santana Luquine Embrapa Cassava & Fruits, Empresa Brasileira de Pesquisa Agropecuária, Cruz das Almas, BA, Brazil https://orcid.org/0000-0002-7254-1039
  • Felipe Caldas Campos Center of Agrarian, Environmental and Biological Sciences, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil https://orcid.org/0009-0000-4985-9818
  • Vinicius dos Reis Aragão Center of Agrarian, Environmental and Biological Sciences, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil https://orcid.org/0009-0003-1632-6646
  • Rone do Carmo Souza Center of Agrarian, Environmental and Biological Sciences, Universidade Federal do Recôncavo da Bahia, Cruz das Almas, BA, Brazil https://orcid.org/0009-0004-1117-0213
  • Leandro de Souza Rocha Embrapa Cassava & Fruits, Empresa Brasileira de Pesquisa Agropecuária, Cruz das Almas, BA, Brazil https://orcid.org/0000-0002-8759-4846
  • Fernando Haddad Embrapa Cassava & Fruits, Empresa Brasileira de Pesquisa Agropecuária, Cruz das Almas, BA, Brazil https://orcid.org/0000-0003-0332-3270

DOI:

https://doi.org/10.1590/1983-21252026v3915036rc

Keywords:

Meloidogyne spp.. Radopholus similis. Bacillus siamensis. Trichoderma asperellum. integrated management.

Abstract

Banana production is affected by plant-health challenges caused by infestations of plant-parasitic nematodes such as Meloidogyne incognita, Meloidogyne enterolobii, and Radopholus similis, which impair root development, yield, and plantation longevity. The efficacy of cyclobutrifluram and its physiological compatibility with biocontrol microorganisms were evaluated under in vitro conditions using a sequential and integrated approach. In Experiment I, the nematicidal activity of cyclobutrifluram was evaluated at different concentrations; in Experiment II, the effects of Bacillus siamensis (CNPMF1009), B. pumilus (BR12158), B. methylotrophicus, B. velezensis (BR12157), and Bacillus sp. (BSF66) strains on plant-parasitic nematodes were determined; in Experiment III, physiological compatibility between cyclobutrifluram and beneficial microorganisms (B. siamensis (CNPMF1009), B. velezensis (BR12157), and Trichoderma asperellum (CNPMF 1007)) was analyzed; and in Experiment IV, biofilm formation by Bacillus spp. was evaluated as an indicator of microbial adaptation and survival. Cyclobutrifluram showed nematicidal activity and efficacy against M. incognita and M. enterolobii, with mortality greater than 90%, as well as activity against R. similis. The Bacillus siamensis and B. velezensis strains also showed nematicidal activity and maintained physiological compatibility with the product at the tested concentrations, whereas T. asperellum growth was reduced at specific product concentrations, indicating greater sensitivity than that of the bacterial strains. Most bacterial strains showed biofilm-forming capacity, indicating potential for adaptation and survival under in vitro conditions. The results showed nematicidal activity and physiological compatibility among cyclobutrifluram, B. siamensis (CNPMF1009), and B. velezensis (BR12157).

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Published

04-08-2026

Issue

Vol. 39 (2026)

Section

Scientific Article

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